PGPEP1 Polyclonal antibody

PGPEP1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 19566-1-AP

Synonyms

5 oxoprolyl peptidase, PAP I, Pcp, PGP, PGP I, PGPEP1, PGPI, Pyroglutamyl aminopeptidase I, Pyroglutamyl peptidase 1, pyroglutamyl peptidase I

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Validation Data Gallery

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  WB analysis of human brain using 19566-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19566-1-AP (PGPEP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 19566-1-AP

  Immunofluorescent analysis of HepG2 cells, using PGPEP1 antibody 19566-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: human brain tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19566-1-AP targets PGPEP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: pyroglutamyl-peptidase I
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 20-23 kDa
• GenBank Accession Number: NM_017712
• Gene Symbol: PGPEP1
• Gene ID (NCBI): 54858
• RRID: AB_10641046
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PGPEP1, also named as PGPI, belongs to the peptidase C15 family. PGPEP1 removes 5-oxoproline from various penultimate amino acid residues except L-proline.