PHACTR3 Polyclonal antibody

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  IP experiment of HL-60 using 18107-1-AP

  IP result of anti-PHACTR3 (IP:18107-1-AP, 4ug; Detection:18107-1-AP 1:1500) with HL-60 cells lysate 2240 ug.

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  IHC staining of mouse brain using 18107-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18107-1-AP (PHACTR3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 18107-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18107-1-AP (PHACTR3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PHACTR3 Polyclonal Antibody for IHC, IP, ELISA

Cat No. 18107-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IP, ELISA

Synonyms

C20orf101, H17739, Scaffold-associated PP1-inhibiting protein, SCAPIN1, Scapinin

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Tested Applications

• Positive IP detected in: HL-60 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18107-1-AP targets PHACTR3 in IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12486

  Product name: Recombinant human PHACTR3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 211-559 aa of BC108303

  Sequence: ADSLDSPPRPLERSVGQLPSPPLLPTPPPKASSKTTKNVTGQATLFQASSMKSADPSLRGQLSTPTGSPHLTTVHRPLPPSRVIEELHRALATKHRQDSFQGRESKGSPKKRLDVRLSRTSSVERGKEREEAWSFDGALENKRTAAKESEENKENLIINSELKDDLLLYQDEEALNDSIISGTLPRKCKKELLAVKLRNRPSKQELEDRNIFPRRTDEERQEIRQQIEMKLSKRLSQRPAVEELERRNILKQRNDQTEQEERREIKQRLTRKLNQRPTVDELRDRKILIRFSDYVEVAKAQDYDRRADKPWTRLSAADKAAIRKELNEYKSNEMEVHASSKHLTRFHRP

• Full Name: phosphatase and actin regulator 3
• Calculated Molecular Weight: 559 aa, 63 kDa
• Observed Molecular Weight: 60 kDa and 75 kDa
• GenBank Accession Number: BC108303
• Gene Symbol: PHACTR3
• Gene ID (NCBI): 116154
• RRID: AB_3669291
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96KR7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PHACTR3 was originally named as the nuclear scaffold-associated PP1-inhibiting protein (Scapinin), which is found in the nuclear insoluble fraction of the leukemia cell line HL-60. However, PHACTR3 is distributed to the plasma membrane in adherent cells, and it enhances cell migration (PMID: 25405772). In humans, there are two PHACTR3 isoforms that are 75 kDa and 60 kDa, respectively (PMID: 32423795).