Tested Applications
| Positive WB detected in | CHO cells, ROS1728 cells, mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
30668-1-AP targets PHEX in WB, ELISA applications and shows reactivity with human, mouse, rat, hamster samples.
| Tested Reactivity | human, mouse, rat, hamster |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag33428 Product name: Recombinant human PHEX protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 430-641 aa of BC105059 Sequence: DKKEMMEELVEGVRWAFIDMLEKENEWMDAGTKRKAKEKARAVLAKVGYPEFIMNDTHVNEDLKAIKFSEADYFGNVLQTRKYLAQSDFFWLRKAVPKTEWFTNPTTVNAFYSASTNQIRFPAGELQKPFFWGTEYPRSLSYGAIGVIVGHEFTHGFDNNGRKYDKNGNLDPWWSTESEEKFKEKTKCMINQYSNYYWKKAGLNVKGKRTLG Predict reactive species |
| Full Name | phosphate regulating endopeptidase homolog, X-linked |
| Calculated Molecular Weight | 749 aa, 86 kDa |
| Observed Molecular Weight | 76-80 kDa, 95-100 kDa |
| GenBank Accession Number | BC105059 |
| Gene Symbol | PHEX |
| Gene ID (NCBI) | 5251 |
| RRID | AB_3742364 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P78562 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Phex encodes a 100- to 105-kDa transmembrane glycoprotein, which is present in bones and teeth of normal mice but not Hyp animals. Phex protein expression in femur and calvaria decreases with age, high levels of Phex detected in osteocytes make this protein an interesting marker to study the later stages of osteoblastic cell differentiation (PMID: 10934642). The 76 kDa represents the deglycosylated core-glycosylated forms within the ERs, the 95 kDa represents the core-glycosylated and unmatured form within the ERs, the 105 kDa species of PHEX protein represents the fully processed, matured for(PMID: 3251189, 35654784).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PHEX antibody 30668-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
