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  • KD/KO Validated

PHF11 Polyclonal antibody

PHF11 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10898-1-AP

Synonyms

APY, IGEL, IGER, IGHER, NY REN 34, NYREN34, PHD finger protein 11, PHF11



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, mouse thymus tissue
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10898-1-AP targets PHF11 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHF11 fusion protein Ag1318
Full Name PHD finger protein 11
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC017212
Gene Symbol PHF11
Gene ID (NCBI) 51131
RRIDAB_2165207
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PHF11 is a transcriptional co-activator of the Th1 effector cytokine genes, interleukin-2 (IL2) and interferon-γ (IFNG), co-operating with nuclear factor kappa B (NF-κB). The reduction in PHF11 expression seen with an AD-associated genotype could contribute to the strong T(H)2 responses that characterize many allergic individuals.

Protocols

Product Specific Protocols
WB protocol for PHF11 antibody 10898-1-APDownload protocol
IHC protocol for PHF11 antibody 10898-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

Mutant Huntingtin stalls ribosomes and represses protein synthesis in a cellular model of Huntington disease.

Authors - Mehdi Eshraghi
humanWB,IP

J Allergy Clin Immunol

Functional characterization of the atopy-associated gene PHF11.

Authors - Clarke Emily E
humanWB

PLoS Pathog

Inhibition of spumavirus gene expression by PHF11.

Authors - Melissa Kane
mouseWB

iScience

Analysis of microbiota reveals the underlying mechanism of PHF11 in the development of Enterococcus-regulated endometriotic cysts

Authors - Jingjing Hu
humanWB

Immunol Cell Biol

A role for the atopy-associated gene PHF11 in T-cell activation and viability.

Authors - Rahman Nusrat N
  • KD Validated
humanIF

BMC Immunol

PHF11 expression and cellular distribution is regulated by the Toll-Like Receptor 3 Ligand Polyinosinic:Polycytidylic Acid in HaCaT keratinocytes.

Authors - Pauline Muscat
  • KD Validated