Tested Applications
| Positive WB detected in | Jurkat cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24624-1-AP targets PHF2 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20257 Product name: Recombinant human PHF2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 445-780 aa of BC152437 Sequence: ASKAVRPEVNTVASSDEVCDGDREKEEPPSPIEATPPQSLLEKVSKKKTPKTVKMPKPSKIPKPPKPPKPPRPPKTLKLKDGGKKKGKKSRESASPTIPNLDLLEAHTKEALTKMEPPKKGKATKSVLSVPNKDVVHMQNDVERLEIREQTKSKSEAKWKYKNSKPDSLLKMEEEQKLEKSPLAGNKDNKFSFSFSNKKLLGSKALRPPTSPGVFGALQNFKEDKPKPVRDEYEYVSDDGELKIDEFPIRRKKNAPKRDLSFLLDKKAVLPTPVTKPKLDSAAYKSDDSSDEGSLHIDTDTKPGRNARVKKESGSSAAGILDLLQASEEVGALEYN Predict reactive species |
| Full Name | PHD finger protein 2 |
| Calculated Molecular Weight | 1101 aa, 121 kDa |
| Observed Molecular Weight | 150 kDa |
| GenBank Accession Number | BC152437 |
| Gene Symbol | PHF2 |
| Gene ID (NCBI) | 5253 |
| RRID | AB_2879644 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75151 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PHD finger protein 2 (PHF2), also known as JHDM1E (Jumonji C domain-containing histone demethylase 1E) or GRC5, is a 1101 amino acid protein, which contains one JmjC domain and one PHD-type zinc finger. PHF2 belongs to the JHDM1 histone demethylase family and localizes in the nucleus. PHF2 demethylases both histones and non-histione proteins. PHF2 is widely expressed in various tissues and exists as two alternative splicing isoforms. The gene encoding PHF2 lies in the candidate region for hereditary sensory neuropathy type I (HSN1), a disorder characterized by sensory dysfunction
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PHF2 antibody 24624-1-AP | Download protocol |
| IP protocol for PHF2 antibody 24624-1-AP | Download protocol |
| WB protocol for PHF2 antibody 24624-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PHF2 Polyclonal antibody (Cat# 24624-1-AP) has 1 citation. It was cited in American Journal of Human Genetics (IF 7.7, 2023), in a study on high-throughput identification of regulatory elements and susceptibility genes for nasopharyngeal carcinoma. The research field is human genetics and cancer genomics. The antibody was applied in Western Blot (WB) with human samples.
| Species | Application | Title |
|---|---|---|
Am J Hum Genet High-throughput identification of regulatory elements and functional assays to uncover susceptibility genes for nasopharyngeal carcinoma |
