PHF23 Polyclonal antibody, PBS Only

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• PHF23 Polyclonal antibody, PBS Only (25862-1-PBS)

• 

  WB analysis using 25862-1-AP (same clone as 25862-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25862-1-AP (PHF23 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25862-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 25862-1-AP (same clone as 25862-1-PBS)

  WB result of PHF23 antibody (25862-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PHF23 transfected HeLa cells. This data was developed using the same antibody clone with 25862-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 25862-1-AP (same clone as 25862-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using PHF23 antibody (25862-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 25862-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PHF23 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 25862-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

PDH-containing protein JUNE-1, hJUNE-1b, hJUNE 1b

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Product Information

25862-1-PBS targets PHF23 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag23044

  Product name: Recombinant human PHF23 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-403 aa of BC002509

  Sequence: MLEAMAEPSPEDPPPTLKPETQPPEKRRRTIEDFNKFCSFVLAYAGYIPPSKEESDWPASGSSSPLRGESAADSDGWDSAPSDLRTIQTFVKKAKSSKRRAAQAGPTQPGPPRSTFSRLQAPDSATLLEKMKLKDSLFDLDGPKVASPLSPTSLTHTSRPPAALTPVPLSQGDLSHPPRKKDRKNRKLGPGAGAGFGVLRRPRPTPGDGEKRSRIKKSKKRKLKKAERGDRLPPPGPPQAPPSDTDSEEEEEEEEEEEEEEMATVVGGEAPVPVLPTPPEAPRPPATVHPEGVPPADSESKEVGSTETSQDGDASSSEGEMRVMDEDIMVESGDDSWDLITCYCRKPFAGRPMIECSLCGTWIHLSCAKIKKTNVPDFFYCQKCKELRPEARRLGGPPKSGEP

• Full Name: PHD finger protein 23
• Calculated Molecular Weight: 403 aa, 44 kDa
• Observed Molecular Weight: ~65 kDa
• GenBank Accession Number: BC002509
• Gene Symbol: PHF23
• Gene ID (NCBI): 79142
• RRID: AB_3085824
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BUL5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PHD finger protein 23 (PHF23), also known as hJUNE-1b, belongs to the PHF23 family. As a putative epigenetic reader, the PHD domain of PHF23 specifically binds to tri-methylated lysine 4 of histone H3 (H3K4me3). PHF23 is widely expressed in human tissues and various cell lines and exerts a negative effect on autophagy by promoting the degradation of E3 ligase LRSAM1 (leucine-rich repeat and sterile alpha motif containing 1) (PMID: 32978226, 32380078). The observed molecular weight of PHF23 is ~65 kDa, which may be caused by post-translational modification. The specificity of this antibody was demonstrated by siRNA-mediated knockdown of target protein.