PHF6 Polyclonal antibody

PHF6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 51062-1-AP

Synonyms

PHF 6, PHD-like zinc finger protein, CENP-31, BORJ

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Validation Data Gallery

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  WB analysis using 51062-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51062-1-AP (PHF6 antibody) at dilution of 1:64000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 51062-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 51062-1-AP (PHF6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 51062-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 51062-1-AP (PHF6 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 51062-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 51062-1-AP (PHF6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 51062-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 51062-1-AP (PHF6 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 51062-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 51062-1-AP (PHF6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 51062-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PHF6 antibody (51062-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, K-562 cells
• Positive IHC detected in: human ovary tumor tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

51062-1-AP targets PHF6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PHF6 fusion protein Ag2102
• Full Name: PHD finger protein 6
• Calculated Molecular Weight: 365aa,41 kDa; 312aa,35 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC005994
• Gene Symbol: PHF6
• Gene ID (NCBI): 84295
• RRID: AB_2165369
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PHF6 protein is highly conserved in vertebrates. Structurally, PHF6 protein contains two plant homeodomain (PHD)-like zinc fingers, two nuclear localization sequences and a nucleolar localization sequence, suggesting that PHF6 may play a role in the regulation of transcription. PHD zinc fingers are structurally conserved modules that interact with chromatin or mediate protein-protein interactions. PHF6 associates with subunits of the PAF1 transcription elongation complex including PAF1, LEO1, CTR9 and CDC73.The interaction of PHF6 with the PAF1 complex plays a critical role in PHF6 function in neuronal migration in the cerebral cortex.

Publications

Species	Application	Title
human	WB	Leuk Res; MiR-1306-5p promotes cell proliferation and inhibits cell apoptosis in acute myeloid leukemia by downregulating PHF6 expression.; Authors - Xiang Gao