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PHGDH Recombinant monoclonal antibody, PBS Only (81986-1-PBS)

Filter: AllWBIPIHCFC (Intra)Affinity

WB analysis of HEK-293 using 81986-1-RR (same clone as 81986-1-PBS)
WB result of PHGDH antibody (81986-1-RR; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PHGDH transfected HEK-293 cells. This data was developed using the same antibody clone with 81986-1-PBS in a different storage buffer formulation.
WB analysis using 81986-1-RR (same clone as 81986-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 81986-1-RR (PHGDH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81986-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 81986-1-RR (same clone as 81986-1-PBS)
IP result of anti-PHGDH (IP:81986-1-RR, 4ug; Detection:81986-1-RR 1:10000) with HeLa cells lysate 1760 ug. This data was developed using the same antibody clone with 81986-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 81986-1-RR (same clone as 81986-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81986-1-RR (PHGDH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81986-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 81986-1-RR (same clone as 81986-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81986-1-RR (PHGDH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81986-1-PBS in a different storage buffer formulation.
FC experiment of HepG2 using 81986-1-RR (same clone as 81986-1-PBS)
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PHGDH Recombinant monoclonal antibody (81986-1-RR, Clone:5L16) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 81986-1-PBS in a different storage buffer formulation.
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1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PHGDH Recombinant monoclonal antibody (81986-1-RR, Clone:5L16) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 81986-1-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 81986-1-RR
Biolayer interferometry (BLl) kinetic assays of 81986-1-RR against Human PHGDH were performed. The affinity constant is below 1 pM.

Featured Product
KD/KO Validated

PHGDH Recombinant monoclonal antibody, PBS Only

Name: PHGDH Recombinant monoclonal antibody, PBS Only
Brand: Proteintech
SKU: 81986-1-PBS

PHGDH Uni-rAb^® Recombinant Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA

Cat No. 81986-1-PBS

Clone No.5L16

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), IP, Indirect ELISA

Synonyms

3 PGDH, 3PGDH, 3-PGDH, D-3-phosphoglycerate dehydrogenase, EC:1.1.1.37

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

81986-1-PBS targets PHGDH in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag6445 Product name: Recombinant human PHGDH protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 186-533 aa of BC000303 Sequence: FGVQQLPLEEIWPLCDFITVHTPLLPSTTGLLNDNTFAQCKKGVRVVNCARGGIVDEGALLRALQSGQCAGAALDVFTEEPPRDRALVDHENVISCPHLGASTKEAQSRCGEEIAVQFVDMVKGKSLTGVVNAQALTSAFSPHTKPWIGLAEALGTLMRAWAGSPKGTIQVITQGTSLKNAGNCLSPAVIVGLLKEASKQADVNLVNAKLLVKEAGLNVTTSHSPAAPGEQGFGECLLAVALAGAPYQAVGLVQGTTPVLQGLNGAVFRPEVPLRRDLPLLLFRTQTSDPAMLPTMIGLLAEAGVRLLSYQTSLVSDGETWHVMGISSLLPSLEAWKQHVTEAFQFHF Predict reactive species
Full Name	phosphoglycerate dehydrogenase
Calculated Molecular Weight	57 kDa
Observed Molecular Weight	57 kDa
GenBank Accession Number	BC000303
Gene Symbol	PHGDH
Gene ID (NCBI)	26227
RRID	AB_2935595
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	O43175
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

PHGDH(D-3-phosphoglycerate dehydrogenase) is also named as 3-PGDH, PGDH3 and belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. It catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor. 3-PGDH deficiency is a rare recessive inborn error in the biosynthesis of the amino acid L-serine characterized clinically by congenital microcephaly, psychomotor retardation, and intractable seizures(PMID:19235232 ).