PHKG1 Polyclonal antibody

PHKG1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 16743-1-AP

Synonyms

PHKG, PHKG1

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Validation Data Gallery

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  WB analysis using 16743-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16743-1-AP (PHKG1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 16743-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16743-1-AP (PHKG1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 16743-1-AP

  IP result of anti-PHKG1 (IP:16743-1-AP, 4ug; Detection:16743-1-AP 1:600) with mouse skeletal muscle tissue lysate 4000ug.

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  IHC staining of mouse skeletal muscle using 16743-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16743-1-AP (PHKG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 16743-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16743-1-AP (PHKG1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, rat skeletal muscle tissue
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

16743-1-AP targets PHKG1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PHKG1 fusion protein Ag10032
• Full Name: phosphorylase kinase, gamma 1 (muscle)
• Calculated Molecular Weight: 387 aa, 45 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC051327
• Gene Symbol: PHKG1
• Gene ID (NCBI): 5260
• RRID: AB_1851532
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PHKG1(Phosphorylase b kinase gamma catalytic chain, skeletal muscle isoform) is also named as phosphorylase kinase subunit gamma-1, PHKG and belongs to the CAMK Ser/Thr protein kinase family. Phosphorylase kinase is a crucial glycogenolytic regulatory enzyme. The holoenzymes are composed of 16 subunit proteins containing equimolar ratios of 4 subunit types known as alpha, beta, gamma, and delta. This protein is the skeletal muscle isoform of phosphorylase kinase and kinetic differences exist between the cardiac and adult skeletal muscle enzymes, and the liver and skeletal muscle enzymes are known to be separate isozymes.

Publications

Species	Application	Title
pig	WB	PLoS Genet; A Splice Mutation in the PHKG1 Gene Causes High Glycogen Content and Low Meat Quality in Pig Skeletal Muscle.; Authors - Junwu Ma
pig	WB	J Biol Chem; Circular RNA circMYLK4 shifts energy metabolism from glycolysis to OXPHOS by binding to the calcium channel auxiliary subunit CACNA2D2; Authors - Haigang Cao