PHLDA1 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 85783-1-RR (same clone as 85783-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85783-1-RR (PHLDA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85783-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 85783-1-RR (same clone as 85783-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85783-1-RR (PHLDA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85783-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 85783-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85783-1-RR against Human PHLDA1 were performed. The affinity constant is below 1 pM.

PHLDA1 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 85783-1-PBS

Clone No.243156B7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

Apoptosis-associated nuclear protein, DT1P1B11, PHRIP, Pleckstrin homology-like domain family A member 1, PQ-rich protein

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Product Information

85783-1-PBS targets PHLDA1 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag13125

  Product name: Recombinant human PHLDA1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-259 aa of BC018929

  Sequence: MLESSGCKALKEGVLEKRSDGLLQLWKKKCCILTEEGLLLIPPKQLQHQQQQQQQQQQQQQQPGQGPAEPSQPSGPAVASLEPPVKLKELHFSNMKTVDCVERKGKYMYFTVVMAEGKEIDFRCPQDQGWNAEITLQMVQYKNRQAILAVKSTRQKQQHLVQQQPPSQPQPQPQLQPQPQPQPQPQPQPQSQPQPQPQPKPQPQQLHPYPHPHPHPHSHPHSHPHPHPHPHPHQIPHPHPQPHSQPHGHRLLRSTSNSA

• Full Name: pleckstrin homology-like domain, family A, member 1
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC018929
• Gene Symbol: PHLDA1
• Gene ID (NCBI): 22822
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8WV24
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PHLDA1, also known as PHRIP and TDAG51, is a multifunctional protein involved in various biological processes. It can induce apoptosis in various cell types, including T cells, hippocampal cells, endothelial cells, melanoma cells, and mouse embryonic fibroblasts(PMID: 30207029). PHLDA1 plays a role in inhibiting growth factor signaling and has been implicated in tumor suppression through its ability to repress Akt activity by binding to phosphatidylinositol (PIP) lipids(PMID: 36142223).