PHLDA1 Recombinant antibody

• 

  WB analysis using 85783-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85783-1-RR (PHLDA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85783-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85783-1-RR (PHLDA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 85783-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85783-1-RR against Human PHLDA1 were performed. The affinity constant is below 1 pM.

PHLDA1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85783-1-RR

Clone No.243156B7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Apoptosis-associated nuclear protein, DT1P1B11, PHRIP, Pleckstrin homology-like domain family A member 1, PQ-rich protein

---

Tested Applications

• Positive WB detected in: MDA-MB-231 cells, HeLa cells, BxPC-3 cells, A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85783-1-RR targets PHLDA1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PHLDA1 fusion protein Ag13125
• Full Name: pleckstrin homology-like domain, family A, member 1
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC018929
• Gene Symbol: PHLDA1
• Gene ID (NCBI): 22822
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8WV24
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PHLDA1, also known as PHRIP and TDAG51, is a multifunctional protein involved in various biological processes. It can induce apoptosis in various cell types, including T cells, hippocampal cells, endothelial cells, melanoma cells, and mouse embryonic fibroblasts(PMID: 30207029). PHLDA1 plays a role in inhibiting growth factor signaling and has been implicated in tumor suppression through its ability to repress Akt activity by binding to phosphatidylinositol (PIP) lipids(PMID: 36142223).