PHOX2B Polyclonal antibody

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  WB analysis of Neuro-2a using 25276-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25276-1-AP (PHOX2B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 25276-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25276-1-AP (PHOX2B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 25276-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25276-1-AP (PHOX2B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of neuroblastoma using 25276-1-AP

  Immunohistochemical analysis of paraffin-embedded neuroblastoma tissue slide using 25276-1-AP (PHOX2B Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse brain using 25276-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25276-1-AP (PHOX2B Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of mouse brain using 25276-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25276-1-AP (PHOX2B Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of neuroblastoma using 25276-1-AP

  Immunohistochemical analysis of paraffin-embedded neuroblastoma tissue slide using 25276-1-AP (PHOX2B Antibody) at dilution of 1:200 (under 10x lens).

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  IF Staining of SH-SY5Y using 25276-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using PHOX2B antibody (25276-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  IF Staining of SH-SY5Y using 25276-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using 25276-1-AP (PHOX2B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

PHOX2B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 25276-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

NBPhox, Neuroblastoma Phox, paired like homeobox 2b, Paired-like homeobox 2B, PMX2B

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Tested Applications

• Positive WB detected in: Neuro-2a cells, SH-SY5Y cells
• Positive IHC detected in: neuroblastoma tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

25276-1-AP targets PHOX2B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13196

  Product name: Recombinant human PHOX2B protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 63-314 aa of BC017199

  Sequence: GSCSLGTLRDHQSSPYAAVPYKLFTDHGGLNEKRKQRRIRTTFTSAQLKELERVFAETHYPDIYTREELALKIDLTEARVQVWFQNRRAKFRKQERAAAAAAAAAKNGSSGKKSDSSRDDESKEAKSTDPDSTGGPGPNPNPTPSCGANGGGGGGPSPAGAPGAAGPGGPGGEPGKGGAAAAAAAAAAAAAAAAAAAAGGLAAAGGPGQGWAPGPGPITSIPDSLGGPFASVLSSLQRPNGAKAALVKSSMF

• Full Name: paired-like homeobox 2b
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC017199
• Gene Symbol: PHOX2B
• Gene ID (NCBI): 8929
• RRID: AB_2880001
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99453
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Paired mesoderm homeobox protein 2B (PHOX2B), also named as Neuroblastoma Phox, is a 314 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the paired homeobox family. PHOX2B localizes in the nucleus and is expressed in neuroblastoma, brain and adrenal gland. PHOX2B plays a role in the development of several major noradrenergic neuron populations, including the locus coeruleus. PHOX2B has an association with congenital central hypoventilation syndrome.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB	Stem Cells Transl Med; Neural Stem Cell-Derived Exosomal Netrin1 Contributes to Neuron Differentiation of Mesenchymal Stem Cells in Therapy of Spinal Bifida Aperta.; Authors - Ling Ma
mouse	IHC	Mol Metab; Ablation of intact hypothalamic and/or hindbrain TrkB signaling leads to perturbations in energy balance.; Authors - Ceren Ozek
human	IF	Sci Rep; Generating trunk neural crest from human pluripotent stem cells.; Authors - Miller Huang
human	IF	Sci Rep; Selective Induction of Human Autonomic Neurons Enables Precise Control of Cardiomyocyte Beating.; Authors - Yuzo Takayama
human	IHC	Int J Mol Sci; Deciphering the Molecular Mechanisms of Autonomic Nervous System Neuron Induction through Integrative Bioinformatics Analysis; Authors - Yuzo Takayama