PHOX2B Polyclonal antibody

PHOX2B Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 25276-1-AP

Synonyms

NBPhox, Neuroblastoma Phox, paired like homeobox 2b, PHOX2B, PHOX2B homeodomain protein, PMX2B



Tested Applications

Positive WB detected inNeuro-2a cells, SH-SY5Y cells
Positive IHC detected inneuroblastoma tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNeuro-2a cells, SH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25276-1-AP is PHOX2B Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHOX2B fusion protein Ag13196
Full Name paired-like homeobox 2b
Calculated molecular weight 31 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC017199
Gene symbol PHOX2B
Gene ID (NCBI) 8929
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Paired mesoderm homeobox protein 2B (PHOX2B), also named as Neuroblastoma Phox, is a 314 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the paired homeobox family. PHOX2B localizes in the nucleus and is expressed in neuroblastoma, brain and adrenal gland. PHOX2B plays a role in the development of several major noradrenergic neuron populations, including the locus coeruleus. PHOX2B has an association with congenital central hypoventilation syndrome.

Protocols

Product Specific Protocols
WB protocol for PHOX2B antibody 25276-1-APDownload protocol
IHC protocol for PHOX2B antibody 25276-1-APDownload protocol
IF protocol for PHOX2B antibody 25276-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Sci Rep

Selective Induction of Human Autonomic Neurons Enables Precise Control of Cardiomyocyte Beating.

Authors - Yuzo Takayama
humanIF

Sci Rep

Generating trunk neural crest from human pluripotent stem cells.

Authors - Miller Huang
mouseIHC

Mol Metab

Ablation of intact hypothalamic and/or hindbrain TrkB signaling leads to perturbations in energy balance.

Authors - Ceren Ozek