Tested Applications
| Positive WB detected in | Neuro-2a cells, SH-SY5Y cells |
| Positive IHC detected in | neuroblastoma tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25276-1-AP targets PHOX2B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13196 Product name: Recombinant human PHOX2B protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 63-314 aa of BC017199 Sequence: GSCSLGTLRDHQSSPYAAVPYKLFTDHGGLNEKRKQRRIRTTFTSAQLKELERVFAETHYPDIYTREELALKIDLTEARVQVWFQNRRAKFRKQERAAAAAAAAAKNGSSGKKSDSSRDDESKEAKSTDPDSTGGPGPNPNPTPSCGANGGGGGGPSPAGAPGAAGPGGPGGEPGKGGAAAAAAAAAAAAAAAAAAAAGGLAAAGGPGQGWAPGPGPITSIPDSLGGPFASVLSSLQRPNGAKAALVKSSMF Predict reactive species |
| Full Name | paired-like homeobox 2b |
| Calculated Molecular Weight | 31 kDa |
| Observed Molecular Weight | 31 kDa |
| GenBank Accession Number | BC017199 |
| Gene Symbol | PHOX2B |
| Gene ID (NCBI) | 8929 |
| RRID | AB_2880001 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99453 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Paired mesoderm homeobox protein 2B (PHOX2B), also named as Neuroblastoma Phox, is a 314 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the paired homeobox family. PHOX2B localizes in the nucleus and is expressed in neuroblastoma, brain and adrenal gland. PHOX2B plays a role in the development of several major noradrenergic neuron populations, including the locus coeruleus. PHOX2B has an association with congenital central hypoventilation syndrome.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PHOX2B antibody 25276-1-AP | Download protocol |
| IHC protocol for PHOX2B antibody 25276-1-AP | Download protocol |
| WB protocol for PHOX2B antibody 25276-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PHOX2B Polyclonal antibody (Cat# 25276-1-AP) has 6 citations published in Molecular Metabolism, Stem Cells Translational Medicine, International Journal of Molecular Sciences, Scientific Reports (×2), and iScience. The research fields span neural development, autonomic nervous system neuron induction, neural crest differentiation, energy balance regulation, and cardiomyocyte control, with applications in IHC, WB, and IF across human, mouse, and rat species.
| Species | Application | Title |
|---|---|---|
Mol Metab Ablation of intact hypothalamic and/or hindbrain TrkB signaling leads to perturbations in energy balance. | ||
Stem Cells Transl Med Neural Stem Cell-Derived Exosomal Netrin1 Contributes to Neuron Differentiation of Mesenchymal Stem Cells in Therapy of Spinal Bifida Aperta. | ||
Int J Mol Sci Deciphering the Molecular Mechanisms of Autonomic Nervous System Neuron Induction through Integrative Bioinformatics Analysis | ||
Sci Rep Selective Induction of Human Autonomic Neurons Enables Precise Control of Cardiomyocyte Beating. | ||
iScience Epigenomic regulation of neural crest differentiation in human-induced pluripotent stem cells. |
