PI4K2B Polyclonal antibody

PI4K2B Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 15074-1-AP

Synonyms

PI 4 Kinase type 2 beta, PI4K2B, PI4KII BETA, PI4KIIB, PIK42B

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Validation Data Gallery

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  WB analysis of HepG2 using 15074-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15074-1-AP (PI4K2B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 15074-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15074-1-AP (PI4K2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 15074-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15074-1-AP (PI4K2B antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 15074-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15074-1-AP (PI4K2B antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 15074-1-AP

  Immunofluorescent analysis of HepG2 cells, using PI4K2B antibody 15074-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 15074-1-AP

  Immunofluorescent analysis of HepG2 cells, using PI4K2B antibody 15074-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse kidney tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

15074-1-AP targets PI4K2B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PI4K2B fusion protein Ag7120
• Full Name: phosphatidylinositol 4-kinase type 2 beta
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 50-54 kDa
• GenBank Accession Number: BC051749
• Gene Symbol: PI4K2B
• Gene ID (NCBI): 55300
• RRID: AB_2163903
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	EMBO J; Defining the proximal interaction networks of Arf GTPases reveals a mechanism for the regulation of PLD1 and PI4KB.; Authors - Zhengming Wu
human	WB	Cell Death Dis; FKBP51 plays an essential role in Akt ubiquitination that requires Hsp90 and PHLPP; Authors - Martina Tufano
human	IHC	BMC Med Genomics; The identification of a two-gene prognostic model based on cisplatin resistance-related ceRNA network in small cell lung cancer; Authors - Yani Zhang