PI4K2B Polyclonal antibody

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• PI4K2B Polyclonal antibody (15074-1-AP)

PI4K2B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15074-1-AP Copied!

Publications (3)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PI4KII-BETA, PI4KIIB, PI4KII BETA, Phosphatidylinositol 4-kinase type II-beta, Phosphatidylinositol 4-kinase type 2-beta

• 

  WB analysis of HepG2 using 15074-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15074-1-AP (PI4K2B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse kidney using 15074-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15074-1-AP (PI4K2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 15074-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15074-1-AP (PI4K2B antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 15074-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15074-1-AP (PI4K2B antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 15074-1-AP

  Immunofluorescent analysis of HepG2 cells, using PI4K2B antibody 15074-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 15074-1-AP

  Immunofluorescent analysis of HepG2 cells, using PI4K2B antibody 15074-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse kidney tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15074-1-AP targets PI4K2B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7120

  Product name: Recombinant human PI4K2B protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 132-481 aa of BC051749

  Sequence: GSYFVKDPKRKIIGVFKPKSEEPYGQLNPKWTKYVHKVCCPCCFGRGCLIPNQGYLSEAGAYLVDNKLHLSIVPKTKVVWLVSETFNYNAIDRAKSRGKKYALEKVPKVGRKFHRIGLPPKIGSFQLFVEGYKEAEYWLRKFEADPLPENIRKQFQSQFERLVILDYIIRNTDRGNDNWLVRYEKQKCEKEIDHKESKWIDDEEFLIKIAAIDNGLAFPFKHPDEWRAYPFHWAWLPQAKVPFSEEIRNLILPYISDMNFVQDLCEDLYELFKTDKGFDKATFESQMSVMRGQILNLTQALRDGKSPFQLVQIPCVIVERSQGGSQGRIVHLSNSFTQTVNCRKPFFSSW

• Full Name: phosphatidylinositol 4-kinase type 2 beta
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 50-54 kDa
• GenBank Accession Number: BC051749
• Gene Symbol: PI4K2B
• Gene ID (NCBI): 55300
• RRID: AB_2163903
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8TCG2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The PI4K2B Polyclonal antibody (Cat# 15074-1-AP) has accumulated 3 citations across Cell Death Dis, EMBO J, and BMC Med Genomics. The cited studies span research fields including Akt ubiquitination regulated by Hsp90 and PHLPP, Arf GTPase interaction networks governing PLD1 and PI4KB regulation, and a two-gene prognostic model based on cisplatin resistance-related ceRNA networks in small cell lung cancer.

Automatically generated from the 3 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Death Dis; FKBP51 plays an essential role in Akt ubiquitination that requires Hsp90 and PHLPP; Authors - Martina Tufano
human	IF	EMBO J; Defining the proximal interaction networks of Arf GTPases reveals a mechanism for the regulation of PLD1 and PI4KB.; Authors - Zhengming Wu
human	IHC	BMC Med Genomics; The identification of a two-gene prognostic model based on cisplatin resistance-related ceRNA network in small cell lung cancer; Authors - Yani Zhang