PICALM Monoclonal antibody, PBS Only

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  WB analysis using 67564-1-Ig (same clone as 67564-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67564-1-Ig (PICALM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67564-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67564-1-Ig (same clone as 67564-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67564-1-Ig (PICALM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67564-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67564-1-Ig (same clone as 67564-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PICALM antibody (67564-1-Ig, Clone: 1F6G7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67564-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67564-1-Ig (same clone as 67564-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PICALM antibody (67564-1-Ig, Clone: 1F6G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67564-1-PBS in a different storage buffer formulation.

PICALM Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 67564-1-PBS

Clone No.1F6G7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

1F6G7, CALM, CLTH, LAP, Phosphatidylinositol-binding clathrin assembly protein

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Product Information

67564-1-PBS targets PICALM in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag29512

  Product name: Recombinant human PICALM protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-356 aa of BC064357

  Sequence: MSGQSLTDRITAAQHSVTGSAVSKTVCKATTHEIMGPKKKHLDYLIQCTNEMNVNIPQLADSLFERTTNSSWVVVFKSLITTHHLMVYGNERFIQYLASRNTLFNLSNFLDKSGLQGYDMSTFIRRYSRYLNEKAVSYRQVAFDFTKVKRGADGVMRTMNTEKLLKTVPIIQNQMDALLDFNVNSNELTNGVINAAFMLLFKDAIRLFAAYNEGIINLLEKYFDMKKNQCKEGLDIYKKFLTRMTRISEFLKVAEQVGIDRGDIPDLSQAPSSLLDALEQHLASLEGKKIKDSTAASRATTLSNAVSSLASTGLSLTKVDEREKQAALEEEQARLKALKEQRLKELAKKPHTSLTT

• Full Name: phosphatidylinositol binding clathrin assembly protein
• Calculated Molecular Weight: 71 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC064357
• Gene Symbol: PICALM
• Gene ID (NCBI): 8301
• RRID: AB_2882778
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q13492
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PICALM (Phosphatidyl Inositol Clathrin Assembly Lymphoid Myeloid protein) is a ubiquitously expressed protein that plays a role in clathrin-mediated endocytosis. PICALM also affects the internalization and trafficking of SNAREs and modulates macroautophagy. PICALM is a highly validated genetic risk factor for Alzheimer's disease (AD). The long isoform of human PICALM has 22 exons (encoding PICALML), while the short isoform lacks exon 14 (encoding PICALMS). (PMID: 33397371, PMID: 26075887, PMID: 26005850)