PIK3C2A Recombinant monoclonal antibody

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• PIK3C2A Recombinant monoclonal antibody (88380-1-RR)

PIK3C2A Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 88380-1-RR

Clone No.260864A5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

EC:2.7.1.137, Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha, Phosphoinositide 3-kinase-C2-alpha, PI3 K C2A, PI3K C2 alpha

• 

  WB analysis using 88380-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 88380-1-RR (PIK3C2A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 88380-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PIK3C2A antibody (88380-1-RR, Clone: 260864A5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, PC-3 cells, NIH/3T3 cells, C6 cells, mouse testis tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

88380-1-RR targets PIK3C2A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag16894

  Product name: Recombinant human PIK3C2A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-338 aa of BC113658

  Sequence: MAQISSNSGFKECPSSHPEPTRAKDVDKEEALQMEAEALAKLQKDRQVTDNQRGFELSSSTRKKAQVYNKQDYDLMVFPESDSQKRALDIDVEKLTQAELEKLLLDDSFETKKTPVLPVTPILSPSFSAQLYFRPTIQRGQWPPGLPGPSTYALPSIYPSTYSKQAAFQNGFNPRMPTFPSTEPIYLSLPGQSPYFSYPLTPATPFHPQGSLPIYRPVVSTDMAKLFDKIASTSEFLKNGKARTDLEITDSKVSNLQVSPKSEDISKFDWLDLDPLSKPKVDNVEVLDHEEEKNVSSLLAKDPWDAVLLEERSTANCHLERKVNGKSLSVATVTRSQS

• Full Name: phosphoinositide-3-kinase, class 2, alpha polypeptide
• Calculated Molecular Weight: 1686 aa, 191 kDa
• Observed Molecular Weight: 191 kDa
• GenBank Accession Number: BC113658
• Gene Symbol: PIK3C2A
• Gene ID (NCBI): 5286
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O00443
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIK3C2A, also known as PI3K-C2alpha, is a member of phosphatidylinositol -4- phosphate 3- kinase (PI3K) family. Genes in this family play a key role in cell signal transduction. The main function of PIK3C2A is to participate in the metabolism of phosphatidylinositol in cells, specifically, it is responsible for converting phosphatidylinositol -4- phosphate (PIP) into phosphatidylinositol -3,4- diphosphate (PIP2).