CoraLite® Plus 488-conjugated PIK3C2A Recombinant monoclonal antibody

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• CoraLite® Plus 488-conjugated PIK3C2A Recombinant monoclonal antibody (CL488-88380)

PIK3C2A Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL488-88380 Copied!

Clone No. 260864A5

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

3, EC:2.7.1.137, Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha, Phosphoinositide 3-kinase-C2-alpha, PI3 K C2A

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  IF Staining of HeLa using CL488-88380

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 PIK3C2A antibody (CL488-88380, Clone: 260864A5 ) at dilution of 1:200, CL594-phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-88380 targets PIK3C2A in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag16894

  Product name: Recombinant human PIK3C2A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-338 aa of BC113658

  Sequence: MAQISSNSGFKECPSSHPEPTRAKDVDKEEALQMEAEALAKLQKDRQVTDNQRGFELSSSTRKKAQVYNKQDYDLMVFPESDSQKRALDIDVEKLTQAELEKLLLDDSFETKKTPVLPVTPILSPSFSAQLYFRPTIQRGQWPPGLPGPSTYALPSIYPSTYSKQAAFQNGFNPRMPTFPSTEPIYLSLPGQSPYFSYPLTPATPFHPQGSLPIYRPVVSTDMAKLFDKIASTSEFLKNGKARTDLEITDSKVSNLQVSPKSEDISKFDWLDLDPLSKPKVDNVEVLDHEEEKNVSSLLAKDPWDAVLLEERSTANCHLERKVNGKSLSVATVTRSQS

• Full Name: phosphoinositide-3-kinase, class 2, alpha polypeptide
• Calculated Molecular Weight: 1686 aa, 191 kDa
• Observed Molecular Weight: 191 kDa
• GenBank Accession Number: BC113658
• Gene Symbol: PIK3C2A
• Gene ID (NCBI): 5286
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Excitation Laser: Blue laser (488 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O00443
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PIK3C2A, also known as PI3K-C2alpha, is a member of phosphatidylinositol -4- phosphate 3- kinase (PI3K) family. Genes in this family play a key role in cell signal transduction. The main function of PIK3C2A is to participate in the metabolism of phosphatidylinositol in cells, specifically, it is responsible for converting phosphatidylinositol -4- phosphate (PIP) into phosphatidylinositol -3,4- diphosphate (PIP2).