PINK1 Polyclonal antibody, PBS Only

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  WB analysis of HeLa using 23274-1-AP (same clone as 23274-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 23274-1-AP (PINK1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23274-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 23274-1-AP (same clone as 23274-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 23274-1-AP (PINK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23274-1-PBS in a different storage buffer formulation.

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  WB analysis using 23274-1-AP (same clone as 23274-1-PBS)

  Non-treated and CCCP treated HeLa cells were subjected to SDS PAGE followed by western blot with 23274-1-AP (PINK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23274-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 23274-1-AP (same clone as 23274-1-PBS)

  WB result of PINK1 antibody (23274-1-AP, 1:600) with si-Control and si-PINK1 transfected HeLa cells. This data was developed using the same antibody clone with 23274-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 23274-1-AP (same clone as 23274-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23274-1-AP (PINK1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23274-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 23274-1-AP (same clone as 23274-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23274-1-AP (PINK1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23274-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 23274-1-AP (same clone as 23274-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PINK1 antibody (23274-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23274-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 23274-1-AP (same clone as 23274-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PINK1 antibody (23274-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23274-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 23274-1-AP (same clone as 23274-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using PINK1 antibody (23274-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23274-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 23274-1-AP (same clone as 23274-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using PINK1 antibody (23274-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23274-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PINK1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 23274-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

BRPK, EC:2.7.11.1, PARK6, PTEN induced putative kinase 1, PTEN-induced putative kinase protein 1

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Product Information

23274-1-PBS targets PINK1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19825

  Product name: Recombinant human PINK1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 336-581 aa of BC028215

  Sequence: PRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNILVELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNGCLMAPEVSTARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQEAQLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGEHILALKNLKLDKMVGWLLQQSAATLLANRLTEKCCVETKMKMLFLANLECETLCQAALLLCSWRAAL

• Full Name: PTEN induced putative kinase 1
• Calculated Molecular Weight: 581 aa, 63 kDa
• Observed Molecular Weight: 65 kDa, 45 kDa
• GenBank Accession Number: BC028215
• Gene Symbol: PINK1
• Gene ID (NCBI): 65018
• RRID: AB_2879244
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q9BXM7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PINK1 is a mitochondrial serine/threonine-protein kinase that protects cells from stress-induced mitochondrial dysfunction. The precursor of PINK1 (65 kDa) is synthesized in the cytosol and is imported into the outer membrane of mitochondria. PINK1 is further transferred into the inner membrane. The full-length PINK1 can be proteolytically processed into 52-55 kDa and 45-46 kDa forms (PMID: 18221368; 25108683; 18031932). The half-life of the mature form of PINK1 is very short and it was proposed that the proteasome is involved in its degradation (PMID: 23472196). The gene of PINK1 maps to chromosome 1p36.12. Two alternatively spliced variants exist, the shorter isoform (30 kDa) produced by alternative splicing. Mutations in the PINK1 gene cause autosomal recessive early-onset Parkinson's disease.