PINK1 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 81991-4-RR (same clone as 81991-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81991-4-RR (PINK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81991-4-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 81991-4-RR (same clone as 81991-4-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug PINK1 Recombinant antibody (81991-4-RR, Clone:240449E7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 81991-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81991-4-RR

  Biolayer interferometry (BLl) kinetic assays of 81991-4-RR against Human PINK1 were performed. The affinity constant is 125 nM.

PINK1 Uni-rAb^® Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Cat No. 81991-4-PBS

Clone No.240449E7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

240449E7, BRPK, EC:2.7.11.1, PARK6, PTEN induced putative kinase 1

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Product Information

81991-4-PBS targets PINK1 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag19825

  Product name: Recombinant human PINK1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 336-581 aa of BC028215

  Sequence: PRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNILVELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNGCLMAPEVSTARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQEAQLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGEHILALKNLKLDKMVGWLLQQSAATLLANRLTEKCCVETKMKMLFLANLECETLCQAALLLCSWRAAL

• Full Name: PTEN induced putative kinase 1
• Calculated Molecular Weight: 581 aa, 63 kDa
• GenBank Accession Number: BC028215
• Gene Symbol: PINK1
• Gene ID (NCBI): 65018
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BXM7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PINK1 is a mitochondrial serine/threonine-protein kinase that protects cells from stress-induced mitochondrial dysfunction. The precursor of PINK1 (65 kDa) is synthesized in the cytosol and is imported into the outer membrane of mitochondria. PINK1 is further transferred into the inner membrane. The full-length PINK1 can be proteolytically processed into 52-55 kDa and 45-46 kDa forms (PMID: 18221368; 25108683; 18031932). The half-life of the mature form of PINK1 is very short and it was proposed that the proteasome is involved in its degradation (PMID: 23472196). The gene of PINK1 maps to chromosome 1p36.12. Two alternatively spliced variants exist, the shorter isoform (30 kDa) produced by alternative splicing. Mutations in the PINK1 gene cause autosomal recessive early-onset Parkinson's disease.