PINK1 Recombinant antibody, PBS Only (Capture)

• 

  WB analysis using 81991-4-RR (same clone as 81991-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81991-4-RR (PINK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81991-4-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 81991-4-RR (same clone as 81991-4-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug PINK1 Recombinant antibody (81991-4-RR, Clone:240449E7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81991-4-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00404-3

  Cytometric bead array standard curve of MP00404-3, PINK1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 81991-4-PBS. Detection antibody: 81991-2-PBS. Standard: Ag19825. Range: 1.56-100 ng/mL.

• 

  Affinity and Kinetic Characterization of 81991-4-RR

  Biolayer interferometry (BLl) kinetic assays of 81991-4-RR against Human PINK1 were performed. The affinity constant is 125 nM.

PINK1 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 81991-4-PBS

Clone No.240449E7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

PTEN-induced putative kinase protein 1, PTEN induced putative kinase 1, PARK6, EC:2.7.11.1, BRPK

---

Product Information

81991-4-PBS targets PINK1 as part of a matched antibody pair:

MP00404-3: 81991-4-PBS capture and 81991-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PINK1 fusion protein Ag19825
• Full Name: PTEN induced putative kinase 1
• Calculated Molecular Weight: 581 aa, 63 kDa
• GenBank Accession Number: BC028215
• Gene Symbol: PINK1
• Gene ID (NCBI): 65018
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BXM7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.