PINK1 Recombinant monoclonal antibody

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• PINK1 Recombinant monoclonal antibody (81991-4-RR)

PINK1 Uni-rAb^® Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 81991-4-RR Copied!

Clone No. 240449E7

Publications (9)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, FC (Intra), IP, ELISA
For IF, select conjugated version.

Synonyms

240449E7, BRPK, EC:2.7.11.1, PARK6, PTEN induced putative kinase 1

• 

  WB analysis using 81991-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81991-4-RR (PINK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of Jurkat using 81991-4-RR

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug PINK1 Recombinant antibody (81991-4-RR, Clone:240449E7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity and Kinetic Characterization of 81991-4-RR

  Biolayer interferometry (BLl) kinetic assays of 81991-4-RR against Human PINK1 were performed. The affinity constant is 125 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, mouse brain tissue, rat brain tissue
• Positive FC (Intra) detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

81991-4-RR targets PINK1 in WB, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag19825

  Product name: Recombinant human PINK1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 336-581 aa of BC028215

  Sequence: PRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNILVELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNGCLMAPEVSTARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQEAQLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGEHILALKNLKLDKMVGWLLQQSAATLLANRLTEKCCVETKMKMLFLANLECETLCQAALLLCSWRAAL

• Full Name: PTEN induced putative kinase 1
• Calculated Molecular Weight: 581 aa, 63 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC028215
• Gene Symbol: PINK1
• Gene ID (NCBI): 65018
• RRID: AB_3670512
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BXM7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PINK1 is a mitochondrial serine/threonine-protein kinase that protects cells from stress-induced mitochondrial dysfunction. The precursor of PINK1 (65 kDa) is synthesized in the cytosol and is imported into the outer membrane of mitochondria. PINK1 is further transferred into the inner membrane. The full-length PINK1 can be proteolytically processed into 52-55 kDa and 45-46 kDa forms (PMID: 18221368; 25108683; 18031932). The half-life of the mature form of PINK1 is very short and it was proposed that the proteasome is involved in its degradation (PMID: 23472196). The gene of PINK1 maps to chromosome 1p36.12. Two alternatively spliced variants exist, the shorter isoform (30 kDa) produced by alternative splicing. Mutations in the PINK1 gene cause autosomal recessive early-onset Parkinson's disease.

Publications

What published studies show

PINK1 Recombinant monoclonal antibody (240449E7), catalog number 81991-4-RR, has 9 total citations. These appear in journals including Science Advances, Journal of Ethnopharmacology, Mitochondrion, Frontiers in Pharmacology, Journal of Molecular and Cellular Cardiology, Brain Research Bulletin, FASEB Journal, Experimental Dermatology, and Animals. Research fields span mitophagy, mitochondrial biology, cardiac dysfunction, pulmonary fibrosis, depression, preeclampsia, photoaging, and non-alcoholic fatty liver disease.

Automatically generated from the 9 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IP	Sci Adv; A long-lived pool of PINK1 imparts a molecular memory of depolarization-induced activity; Authors - Liam Pollock
		J Ethnopharmacol; Salvia miltiorrhiza Bunge alleviates pulmonary fibrosis by promoting fibroblast mitophagy.; Authors - Xing Zhang
mouse	WB	Mitochondrion; Drp1 knockdown aggravates obesity-induced cardiac dysfunction and remodeling; Authors - Dan Wu
rat	WB	Front Pharmacol; Components of Panax ginseng and Rhodiola rosea regulate mitophagy via the SIRT1/3-PGC-1α-NRF2 pathway to improve myocardial ischemia-reperfusion injury.; Authors - Ming Yao
mouse	WB	J Mol Cell Cardiol; TRPM8-dependent protective effects of L-menthol attenuates lipid overload-induced calcium dysregulation and mitochondrial dysfunction in mouse ventricular myocytes.; Authors - Xiang Sun
rat	WB	Brain Res Bull; Electroacupuncture prevents depression via PINK1/Parkin-driven suppression of NLRP3 activation.; Authors - Zhenhan Li