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PIP4K2A Polyclonal antibody, PBS Only (12469-1-PBS)

Filter: AllWBIPIHCIF/ICC

WB analysis of mouse brain using 12469-1-AP (same clone as 12469-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12469-1-AP (PIP4K2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12469-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 12469-1-AP (same clone as 12469-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12469-1-AP (PIP4K2A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12469-1-PBS in a different storage buffer formulation.
WB analysis using 12469-1-AP (same clone as 12469-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 12469-1-AP (PIP4K2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12469-1-PBS in a different storage buffer formulation.
WB analysis using 12469-1-AP (same clone as 12469-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 12469-1-AP (PIP4K2A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12469-1-PBS in a different storage buffer formulation.
IP experiment of mouse brain using 12469-1-AP (same clone as 12469-1-PBS)
IP result of anti-PIP4K2A (IP:12469-1-AP, 4ug; Detection:12469-1-AP 1:500) with mouse brain tissue lysate 4400ug. This data was developed using the same antibody clone with 12469-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 12469-1-AP (same clone as 12469-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 12469-1-AP (PIP4K2A Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12469-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 12469-1-AP (same clone as 12469-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 12469-1-AP (PIP4K2A Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12469-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 12469-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12469-1-AP (PIP4K2A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 12469-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12469-1-AP (PIP4K2A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 12469-1-AP (same clone as 12469-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PIP4K2A antibody (12469-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (black). This data was developed using the same antibody clone with 12469-1-PBS in a different storage buffer formulation.
IF Staining of SH-SY5Y using 12469-1-AP (same clone as 12469-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using 12469-1-AP (PIP4K2A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12469-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

PIP4K2A Polyclonal antibody, PBS Only

Name: PIP4K2A Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 12469-1-PBS

PIP4K2A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12469-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha, Phosphatidylinositol 5-phosphate 4-kinase type II alpha, Phosphatidylinositol 5-Phosphate 4-Kinase, EC:2.7.1.149, Diphosphoinositide kinase 2-alpha

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

12469-1-PBS targets PIP4K2A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3152 Product name: Recombinant human PIP4K2A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 59-406 aa of BC018034 Sequence: VMLMPDDFKAYSKIKVDNHLFNKENMPSHFKFKEYCPMVFRNLRERFGIDDQDFQNSLTRSAPLPNDSQARSGARFHTSYDKRYIIKTITSEDVAEMHNILKKYHQYIVECHGITLLPQFLGMYRLNVDGVEIYVIVTRNVFSHRLSVYRKYDLKGSTVAREASDKEKAKELPTLKDNDFINEGQKIYIDDNNKKVFLEKLKKDVEFLAQLKLMDYSLLVGIHDVERAEQEEVECEENDGEEEGESDGTHPVGTPPDSPGNTLNSSPPLAPGEFDPNIDVYGIKCHENSPRKEVYFMAIIDILTHYDAKKKAAHAAKTVKHGAGAEISTVNPEQYSKRFLDFIGHILT Predict reactive species
Full Name	phosphatidylinositol-5-phosphate 4-kinase, type II, alpha
Calculated Molecular Weight	406 aa, 46 kDa
Observed Molecular Weight	46 kDa
GenBank Accession Number	BC018034
Gene Symbol	PIP4K2A
Gene ID (NCBI)	5305
RRID	AB_10639518
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P48426
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.