PISD Recombinant monoclonal antibody

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  WB analysis using 84866-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84866-2-RR (PISD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 84866-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84866-2-RR (PISD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 84866-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84866-2-RR against Human PISD were performed. The affinity constant is 0.441 nM.

PISD Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 84866-2-RR

Clone No.242280C9

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

PSDC, Phosphatidylserine decarboxylase proenzyme, mitochondrial, Phosphatidylserine decarboxylase beta chain, Phosphatidylserine decarboxylase alpha chain, EC:4.1.1.65

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Tested Applications

• Positive WB detected in: L02 cells, HSC-T6 cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84866-2-RR targets PISD in WB, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag9499

  Product name: Recombinant human PISD protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-375 aa of BC001482

  Sequence: MMCQSEARQGPELRAAKWLHFPQLALRRRLGQLSCMSRPALKLRSWPLTVLYYLLPFGALRPLSRVGWRPVSRVALYKSVPTRLLSRAWGRLNQVELPHWLRRPVYSLYIWTFGVNMKEAAVEDLHHYRNLSEFFRRKLKPQARPVCGLHSVISPSDGRILNFGQVKNCEVEQVKGVTYSLESFLGPRMCTEDLPFPPAASCDSFKNQLVTREGNELYHCVIYLAPGDYHCFHSPTDWTVSHRRHFPGSLMSVNPGMARWIKELFCHNERVVLTGDWKHGFFSLTAVGATNVGSIRIYFDRDLHTNSPRHSKGSYNDFSFVTHTNREGVPMRKGEHLGEFNLGSTIVLIFEAPKDFNFQLKTGQKIRFGEALGSL

• Full Name: phosphatidylserine decarboxylase
• Calculated Molecular Weight: 375aa,43 kDa; 408aa,46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC001482
• Gene Symbol: PISD
• Gene ID (NCBI): 23761
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UG56
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PISD(Phosphatidylserine decarboxylase proenzyme) belongs to the phosphatidylserine decarboxylase family.It contains a conserved LGST motif that functions as an autocatalytic cleavage site where the proenzyme is split into mature alpha and beta subunits.It has 2 isoforms produced by alternative splicing.