PITRM1 Polyclonal antibody

PITRM1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30674-1-AP

Synonyms

hMP1, hPreP, KIAA1104, Metalloprotease 1, MP1, pitrilysin metallopeptidase 1, Pitrilysin metalloproteinase 1, PITRM1, PreP

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Validation Data Gallery

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  WB analysis using 30674-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30674-1-AP (PITRM1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 30674-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 30674-1-AP (PITRM1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 30674-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 30674-1-AP (PITRM1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, human placenta tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30674-1-AP targets PITRM1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PITRM1 fusion protein Ag30478
• Full Name: pitrilysin metallopeptidase 1
• Calculated Molecular Weight: 60 kDa, 117 kDa
• Observed Molecular Weight: 110-120 kDa
• GenBank Accession Number: BC001150
• Gene Symbol: PITRM1
• Gene ID (NCBI): 10531
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PITRM1 (Presequence protease, mitochondrial) is a ATP-independent protease that degrades mitochondrial transit peptides after their cleavage.It is specific for peptides in the range of 10 to 65 residues.and able to degrade amyloid beta A4 (APP) protein when it accumulates in mitochondrion, suggesting a link with Alzheimer disease.It shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference(PMID:16849325).This protein has a calculated molecular mass of 117kD and it is expressed widely.