Tested Applications
| Positive WB detected in | human testis tissue, mouse testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human testis tissue, human liver cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15659-1-AP targets PIWIL1 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8056 Product name: Recombinant human PIWIL1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-350 aa of BC028581 Sequence: MTGRARARARGRARGQETAQLVGSTASQQPGYIQPRPQPPPAEGELFGRGRQRGTAGGTAKSQGLQISAGFQELSLAERGGRRRDFHDLGVNTRQNLDHVKESKTGSSGIIVRLSTNHFRLTSRPQWALYQYHIDYNPLMEARRLRSALLFQHEDLIGKCHAFDGTILFLPKRLQQKVTEVFSKTRNGEDVRITITLTNELPPTSPTCLQFYNIIFRRLLKIMNLQQIGRNYYNPNDPIDIPSHRLVIWPGFTTSILQYENSIMLCTDVSHKVLRSETVLDFMFNFYHQTEEHKFQEQVSKELIGLVVLTKYNNKTYRVDDIDWDQNPKSTFKKADGSEVSFLEYYRKQY Predict reactive species |
| Full Name | piwi-like 1 (Drosophila) |
| Calculated Molecular Weight | 99 kDa |
| Observed Molecular Weight | 85-95 kDa |
| GenBank Accession Number | BC028581 |
| Gene Symbol | PIWIL1 |
| Gene ID (NCBI) | 9271 |
| RRID | AB_2878163 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96J94 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PIWIL1 antibody 15659-1-AP | Download protocol |
| IHC protocol for PIWIL1 antibody 15659-1-AP | Download protocol |
| IP protocol for PIWIL1 antibody 15659-1-AP | Download protocol |
| WB protocol for PIWIL1 antibody 15659-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PIWIL1 Polyclonal antibody (Cat# 15659-1-AP) has 11 total citations. These appear in journals including Nature Communications, Nature Cardiovascular Research, Journal of Translational Medicine, Development, Molecular Therapy Nucleic Acids, Journal of Cellular and Molecular Medicine, Biology, Prostate, Clinical Genetics, and Andrologia. The associated research fields span germ-line genome integrity, heart regeneration, cervical cancer, spermatogenesis and male infertility, blood-tumor barrier regulation, and prostate epithelium small RNA studies.
| Species | Application | Title |
|---|---|---|
Nat Commun Amniotes co-opt intrinsic genetic instability to protect germ-line genome integrity | ||
Nat Cardiovasc Res The highly conserved PIWI-interacting RNA CRAPIR antagonizes PA2G4-mediated NF110-NF45 disassembly to promote heart regeneration in mice | ||
J Transl Med piRNA-14633 promotes cervical cancer cell malignancy in a METTL14-dependent m6A RNA methylation manner. | ||
Development MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis | ||
Mol Ther Nucleic Acids PIWIL1/piRNA-DQ593109 Regulates the Permeability of the Blood-Tumor Barrier via the MEG3/miR-330-5p/RUNX3 Axis. | ||
J Cell Mol Med Loss-of-function variant in TDRD6 cause male infertility with severe oligo-astheno-teratozoospermia in human and mice |
Reviews
What customers say
The single review for this product describes an IHC experiment on paraffin-embedded adult bovine testis using a 1:200 dilution. The reviewer noted some background noise but confirmed that the positive signal was easily distinguishable. The product received a 4-star rating, indicating overall satisfaction with its performance in immunohistochemistry applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Kelsey (Verified Customer) (12-22-2025) | IHC on paraffin-embedded adult bovine testis. There's some background noise, but the positive signal is easily distinguishable.
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