PIWIL2 Polyclonal antibody

• Featured Product
• KD/KO Validated

PIWIL2 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 14209-1-AP

Synonyms

Cancer/testis antigen 80, CT80, HILI, mili, piwi like 2 (Drosophila), Piwi like protein 2, PIWIL1L, PIWIL2

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Validation Data Gallery

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  IHC staining of human testis using 14209-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14209-1-AP (PIWIL2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 14209-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14209-1-AP (PIWIL2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 14209-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14209-1-AP (PIWIL2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 14209-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14209-1-AP (PIWIL2 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human testis tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

14209-1-AP targets PIWIL2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PIWIL2 fusion protein Ag5423
• Full Name: piwi-like 2 (Drosophila)
• Calculated Molecular Weight: 110 kDa
• GenBank Accession Number: BC025995
• Gene Symbol: PIWIL2
• Gene ID (NCBI): 55124
• RRID: AB_2878028
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Int Med Res; P-element Induced WImpy protein-like RNA-mediated gene silencing 2 regulates tumor cell progression, apoptosis, and metastasis in oral squamous cell carcinoma.; Authors - Sen Zhou ; KD Validated
human	WB,IHC,IF	Cell Oncol (Dordr); Dexmedetomidine promotes colorectal cancer progression via Piwil2 signaling; Authors - Jing Dong ; KD Validated