Tested Applications
| Positive WB detected in | mouse testis tissue, rat testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
34244-1-AP targets PIWIL2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag38831 Product name: Recombinant human PIWIL2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-150 aa of BC025995 Sequence: MDPFRPSFRGQSPIHPSQCQAVRMPGCWPQASKPLDPALGRGAPAGRGHVFGKPEEPSTQRGPAQRESVGLVSMFRGLGIETVSKTPLKREMLPSGRGILGRGLSANLVRKDREELSPTFWDPKVLAAGDSKMAETSVGWSRTLGRGSSD Predict reactive species |
| Full Name | piwi-like 2 (Drosophila) |
| Calculated Molecular Weight | 110 kDa |
| Observed Molecular Weight | 109 kDa |
| GenBank Accession Number | BC025995 |
| Gene Symbol | PIWIL2 |
| Gene ID (NCBI) | 55124 |
| RRID | AB_3743179 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q8TC59 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PIWIL2 is a protein-coding gene that belongs to the Argonaute family of proteins, specifically the Piwi subfamily. The gene shows highly biased expression, found predominantly in the adult testis, with lower levels detected in the duodenum and other tissues. But PIWIL2 is widely expressed in a variety of tumors. PIWIL2 functions as an endoribonuclease ("slicer") that plays a central role in the piRNA (PIWI-interacting RNA) pathway . Its primary role is to protect the germline genome by repressing transposable elements (mobile genetic elements or "jumping genes"), thereby preventing their mobilization which could otherwise cause mutations and disrupt gametogenesis. (PMID: 35794855, PMID: 40789164)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PIWIL2 antibody 34244-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PIWIL2 Polyclonal antibody (Cat# 34244-1-AP) has 1 total citation. It was cited in Burns Trauma (Impact Factor: 11), a 2025 study investigating macrophage-derived exosome piR-50971 in sepsis-induced myocardial injury. The research field encompasses sepsis pathophysiology, cardiac injury, autophagy regulation, and N6-methyladenosine (m6A) RNA modification of mTOR signaling. The antibody was used in Western blot with rat samples.
| Species | Application | Title |
|---|---|---|
Burns Trauma Macrophage-derived exosome piR-50971 exacerbates sepsis-induced myocardial injury by inhibiting autophagy through the upregulation of N6-Methyladenosine modification of mTOR. |
