PIWIL4 Polyclonal antibody

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  WB analysis using 12966-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12966-1-AP (PIWIL4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

PIWIL4 Polyclonal Antibody for WB, ELISA

Cat No. 12966-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

HIWI2, MIWI2, PIWI, piwi like 4 (Drosophila), Piwi like protein 4

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Tested Applications

• Positive WB detected in: human testis tissue, mouse testis tissue, rat testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12966-1-AP targets PIWIL4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PIWIL4 fusion protein Ag3613
• Full Name: piwi-like 4 (Drosophila)
• Calculated Molecular Weight: 852 aa, 97 kDa
• Observed Molecular Weight: 97 kDa
• GenBank Accession Number: BC031060
• Gene Symbol: PIWIL4
• Gene ID (NCBI): 143689
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q7Z3Z4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIWIL4 (PiWI-like RNA-mediated gene silencing 4) is a member of the PIWI protein family and is mainly involved in the development and maintenance of reproductive stem cells. PIWIL4 regulates the biosynthesis and function of piRNA in germ cells by binding to PIWI-interacting RNA (piRNA). Furthermore, PIWIL4 is abnormally expressed in a variety of cancers and plays a promoting role in cancer.