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CoraLite® Plus 647-conjugated PKC Iota Monoclonal antibody
PKC Iota Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG2a
Reactivity
Human, rat, mouse
Applications
FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
3A1B6
Cat no : CL647-66493
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
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Sample-dependent, check data in validation data gallery |
Product Information
CL647-66493 targets PKC Iota in FC (Intra) applications and shows reactivity with Human, rat, mouse samples.
Tested Reactivity | Human, rat, mouse |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | PKC Iota fusion protein Ag4990 |
Full Name | protein kinase C, iota |
Calculated molecular weight | 68 kDa |
Observed molecular weight | 70 kDa |
GenBank accession number | BC022016 |
Gene symbol | PRKCI |
Gene ID (NCBI) | 5584 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The atypical protein kinase C isoform PRKC iota (PRKCI) is a member of the protein kinase C (PKC) family of serine/threonine protein kinases. PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. PRKC iota is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. PRKC iota plays a key role in cell proliferation, differentiation, and carcinogenesis, and it has been shown to be a human oncogene.
Protocols
Product Specific Protocols | |
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FC protocol for CL Plus 647 PKC Iota antibody CL647-66493 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |