PKD1L2 Polyclonal antibody

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  WB analysis using 16333-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16333-1-AP (PKD1L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

PKD1L2 Polyclonal Antibody for WB, ELISA

Cat No. 16333-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Polycystin-1L2, Polycystin 1L2, Polycystic kidney disease protein 1-like 2, PC1-like 2 protein, PC1L2

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Tested Applications

• Positive WB detected in: HEK-293 cells, L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16333-1-AP targets PKD1L2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9455

  Product name: Recombinant human PKD1L2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-306 aa of BC014157

  Sequence: MGEDSPVAMFSWYLDNTPTEQAEPLPDACRLRGFWPRSLTLLQSNTSTLLLNSSFLQSRGEVIRIRATALTRHAYGEDTYVISTVPPREVPACTIAPEEGTVLTSFAIFCNASTALGPLEFCFCLESGSCLHCGPEPALPSVYLPLGEENNDFVLTVVISATNRAGDTQQTQAMAKVALGDTCVEDVAFQAAVSEKIPTALQGEGGPEQLLQLAKAVSSMLNQEHESQGSGQSLSIDVRQKVREHVLGSLSAVTTGLEDVQRVQELAEVLREVTCRSKELTPSAQGSCMGDSWEGAPPAAHVSHAR

• Full Name: polycystic kidney disease 1-like 2
• Calculated Molecular Weight: 306aa,33 kDa; 2459aa,273 kDa
• Observed Molecular Weight: 270 kDa
• GenBank Accession Number: BC014157
• Gene Symbol: PKD1L2
• Gene ID (NCBI): 114780
• RRID: AB_3669239
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q7Z442
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PKD1L2 is a member of the polycystin protein family. PKD1L2 may function as a G-protein-coupled component or regulator of cation channel pores. The long isoform of this protein contains 11 transmembrane domains, a latrophilin/CL-1-like GPCR proteolytic site (GPS) domain, and a polycystin-1, lipoxygenase, alpha-toxin (PLAT) domain.