PKD2 Recombinant monoclonal antibody

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  WB analysis using 82524-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82524-2-RR (PKD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 82524-2-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using PKD2 antibody (82524-2-RR, Clone: 251151C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

PKD2 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 82524-2-RR

Clone No.251151C3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Polycystin 2, APKD2, PC2, PKD4, Polycystin-2

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Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells, HEK-293 cells, Caki-1 cells
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82524-2-RR targets PKD2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: polycystic kidney disease 2 (autosomal dominant)
• Calculated Molecular Weight: 968 aa, 110 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC112263
• Gene Symbol: PKD2
• Gene ID (NCBI): 5311
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13563
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Polycystin 2 (PKD2), the product of the gene mutated in type 2 autosomal dominant polycystic kidney disease, belongs to the polycystin family. PKD2 is a ~110-kDa six-transmembrane channel protein with cytoplasmic N- and C-termini. This protein functions as a Ca2+-activated intracellular Ca2+ release channel in the endoplasmic reticulum. It is also present in the plasma membrane, where it functions as a nonselective cation channel. In addition, PKD2 expression has been documented in the primary cilium of kidney epithelial cells, where it is believed to have an essential role in mediating Ca2+ entry in response to flow rate changes, suggesting that it may be part of a mechanosensing machinery residing in the primary cilium. (PMID: 16135816; 10497221)