PKM Recombinant monoclonal antibody, PBS Only

• Home
• Products
• Primary Antibodies
• Recombinant Antibodies
• PKM Recombinant monoclonal antibody, PBS Only (84451-3-PBS)

PKM Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 84451-3-PBS

Clone No.260211H3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Pyruvate kinase PKM, CTHBP, Cytosolic thyroid hormone-binding protein, EC:2.7.1.40, EC:2.7.10.2

• 

  WB analysis using 84451-3-RR (same clone as 84451-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84451-3-RR (PKM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84451-3-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 84451-3-RR (same clone as 84451-3-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PKM antibody (84451-3-RR, Clone: 260211H3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84451-3-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

---

Product Information

84451-3-PBS targets PKM in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag0110

  Product name: Recombinant human PKM protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 11-221 aa of BC000481

  Sequence: AFIQTQQLHAAMADTFLEHMCRLDIDSPPITARNTGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAV

• Full Name: PKM pyruvate kinase, muscle
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC000481
• Gene Symbol: PKM
• Gene ID (NCBI): 5315
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P14618
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PKM, also named as OIP3, PK2, PK3, PKM1/2, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named as PKM1/M2. The activity of the M2 isoform can be inhibited by tyrosine kinase signalling in tumourcells. The primary pyruvate kinase isoform before tumour development is PKM1; however, the primary isoform from four independent tumours is PKM2.(PMID:18337823). This antibody can recognize both PKM1 and PKM2.