PKM Recombinant monoclonal antibody

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• PKM Recombinant monoclonal antibody (84451-3-RR)

PKM Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 84451-3-RR

Clone No.260211H3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Pyruvate kinase PKM, CTHBP, Cytosolic thyroid hormone-binding protein, EC:2.7.1.40, EC:2.7.10.2

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  WB analysis using 84451-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84451-3-RR (PKM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 84451-3-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PKM antibody (84451-3-RR, Clone: 260211H3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, MCF-7 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84451-3-RR targets PKM in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag0110

  Product name: Recombinant human PKM protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 11-221 aa of BC000481

  Sequence: AFIQTQQLHAAMADTFLEHMCRLDIDSPPITARNTGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAV

• Full Name: PKM pyruvate kinase, muscle
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC000481
• Gene Symbol: PKM
• Gene ID (NCBI): 5315
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P14618
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PKM, also named as OIP3, PK2, PK3, PKM1/2, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named as PKM1/M2. The activity of the M2 isoform can be inhibited by tyrosine kinase signalling in tumourcells. The primary pyruvate kinase isoform before tumour development is PKM1; however, the primary isoform from four independent tumours is PKM2.(PMID:18337823). This antibody can recognize both PKM1 and PKM2.