PLA2G4A Monoclonal antibody

PLA2G4A Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D10H8

Cat no : 68133-1-Ig

Synonyms

cPLA2, cPLA2 alpha, Cytosolic phospholipase A2, PLA2G4, PLA2G4A

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Validation Data Gallery

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  WB analysis using 68133-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68133-1-Ig (PLA2G4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 68133-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 68133-1-Ig (PLA2G4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 68133-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68133-1-Ig (PLA2G4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 68133-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 68133-1-Ig (PLA2G4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 68133-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68133-1-Ig (PLA2G4A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 68133-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68133-1-Ig (PLA2G4A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 68133-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PLA2G4A antibody (68133-1-Ig, Clone: 2D10H8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, NIH/3T3 cells, A549 cells, K-562 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68133-1-Ig targets PLA2G4A in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PLA2G4A fusion protein Ag32674
• Full Name: phospholipase A2, group IVA (cytosolic, calcium-dependent)
• Calculated Molecular Weight: 749 aa, 85 kDa
• Observed Molecular Weight: 85-100 kDa
• GenBank Accession Number: BC114340
• Gene Symbol: PLA2G4A
• Gene ID (NCBI): 5321
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.