PLA2G4D Monoclonal antibody, PBS Only

• 

  WB analysis using 66752-1-Ig (same clone as 66752-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66752-1-Ig (PLA2G4D antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66752-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Caco-2 using 66752-1-Ig (same clone as 66752-1-PBS)

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 66752-1-Ig (PLA2G4D antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66752-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66752-1-Ig (same clone as 66752-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PLA2G4D antibody (66752-1-Ig, Clone: 2E2A10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66752-1-PBS in a different storage buffer formulation.

PLA2G4D Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 66752-1-PBS

Clone No.2E2A10

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

cPLA2 delta, cPLA2delta, Phospholipase A2 group IVD, PLA2G4D

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Product Information

66752-1-PBS targets PLA2G4D in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4178

  Product name: Recombinant human PLA2G4D protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-216 aa of BC034571

  Sequence: MGRLMRRIPEPRICFLEAIWSNIFSLNLLDAWYDLTSSGESWKQHIKDKTRSLEKEPLTTSGTSSRLEASWLQPGTALAQAFKGFLTGRPLHQRSPNFLQGLQLHQDYCSHKDFSTWADYQLDSMPSQLTPKEPRLCLVDAAYFINTSSPSMFRPGRRLDLILSFDYSLSAPFEVPWSPQGNPSAQPGQAPEASSRATEPLPHTARVPKGRRGVRP

• Full Name: phospholipase A2, group IVD (cytosolic)
• Calculated Molecular Weight: 818 aa, 92 kDa
• Observed Molecular Weight: 92 kDa
• GenBank Accession Number: BC034571
• Gene Symbol: PLA2G4D
• Gene ID (NCBI): 283748
• RRID: AB_2882098
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q86XP0
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PLA2G4D(Cytosolic phospholipase A2 delta) belongs to the phospholipase A2 enzyme family,catalyzes the hydrolysis of glycerophospholipids at the sn-2 position and then liberate free fatty acids and lysophospholipids.PLA2G4D expression is specifically upregulated in the upper epidermis of patients with inflammatory skin diseases(PMID:14709560).