iPLA2 Polyclonal antibody

iPLA2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22030-1-AP

Synonyms

CaI PLA2, Group VI phospholipase A2, GVI, GVI PLA2, INAD1, iPLA2, iPLA2 beta, IPLA2 VIA, PARK14, PLA2, PLA2G6, PNPLA9



Tested Applications

Positive WB detected inmouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman small intestine tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22030-1-AP targets iPLA2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen iPLA2 fusion protein Ag16896
Full Name phospholipase A2, group VI (cytosolic, calcium-independent)
Calculated Molecular Weight 90 kDa
Observed Molecular Weight 80 kDa, 88 kDa
GenBank Accession NumberBC036742
Gene Symbol PLA2G6
Gene ID (NCBI) 8398
RRIDAB_2878976
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLA2G6, also named as PLPLA9, is involved in the remodeling of membranes allowing arachidonic acid to be placed in the proper position in phospholipids for stimulated release. It has been implicated in normal phospholipid remodeling, nitric oxide-induced or vasopressin-induced arachidonic acid release and in leukotriene and prostaglandin production. This protein has 4 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for iPLA2 antibody 22030-1-APDownload protocol
IHC protocol for iPLA2 antibody 22030-1-APDownload protocol
IP protocol for iPLA2 antibody 22030-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

Alpha synuclein determines ferroptosis sensitivity in dopaminergic neurons via modulation of ether-phospholipid membrane composition

Authors - Laura Mahoney-Sanchez
human,mouseWB,IHC

J Agric Food Chem

Inhibitory Effects of Peroxidase from Foxtail Millet Bran on Colitis-Associated Colorectal Carcinogenesis by the Blockage of Glycerophospholipid Metabolism.

Authors - Shuhua Shan
ratIF

Neurol Res

Calcium-independent phospholipase A2 inhibitor produces an analgesic effect in a rat model of neuropathic pain by reducing central sensitization in the dorsal horn.

Authors - Young Seob Gwak
humanIHC

Zhongguo Dang Dai Er Ke Za Zhi

[Clinical features of infantile neuroaxonal dystrophy and PLA2G6 gene testing].

Authors - Yao Lu
humanWB,IHC

Theranostics

Ferritin light chain promotes the reprogramming of glioma immune microenvironment and facilitates glioma progression

Authors - Hongjiang Li
humanIHC

Molecules

Identification of Diagnostic Metabolic Signatures in Thyroid Tumors Using Mass Spectrometry Imaging

Authors - Xinxin Mao