PLCB4 Polyclonal antibody

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• PLCB4 Polyclonal antibody (33683-1-AP)

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  WB analysis using 33683-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33683-1-AP (PLCB4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

PLCB4 Polyclonal Antibody for WB, ELISA

Cat No. 33683-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4, EC:3.1.4.11, Phosphoinositide phospholipase C-beta-4, Phospholipase C-beta-4, PLC-beta-4

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Tested Applications

• Positive WB detected in: RAW 264.7 cells, mouse cerebellum tissue, rat cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33683-1-AP targets PLCB4 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag38268

  Product name: Recombinant human PLCB4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 900-1110 aa of NM_182797.2

  Sequence: ALASGVEAKKGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKSTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSEMINTHSAEEQEIRDLHLSQQCELLKKLLINAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVREL

• Full Name: phospholipase C, beta 4
• Calculated Molecular Weight: 134 kDa，1175aa
• Observed Molecular Weight: 134 kDa
• GenBank Accession Number: NM_182797.2
• Gene Symbol: PLCB4
• Gene ID (NCBI): 5332
• RRID: AB_3743049
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q15147
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Phospholipase C beta 4 (PLCB4) is a direct effector of the endothelin receptor signaling pathway that plays an essential role in lower jaw and middle ear structures development (PMID: 35284927). Additionally, PLCB4 has an critical role in osteosarcoma progression and may serve as a novel diagnostic or therapeutic target in pediatric patients with acute myeloid leukemia (PMID: 39987366; 31788080).