PLEC Polyclonal antibody, PBS Only

• Home
• Products
• Primary Antibodies
• PLEC Polyclonal antibody, PBS Only (29170-1-PBS)

• 

  WB analysis using 29170-1-AP (same clone as 29170-1-PBS)

  Various lysates were subjected to Tris-Acetate gel system followed by western blot with 29170-1-AP (PLEC antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29170-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 29170-1-AP (same clone as 29170-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 29170-1-AP (PLEC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29170-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 29170-1-AP (same clone as 29170-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using PLEC antibody (29170-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 29170-1-PBS in a different storage buffer formulation.

PLEC Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 29170-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

PLEC1, Plectin, PLEC1b, Plectin 1, PLTN

---

Product Information

29170-1-PBS targets PLEC in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag29418

  Product name: Recombinant human PLEC protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1475-1768 aa of NM_201380

  Sequence: SETLRRMEEEERLAEQQRAEERERLAEVEAALEKQRQLAEAHAQAKAQAEREAKELQQRMQEEVVRREEAAVDAQQQKRSIQEELQQLRQSSEAEIQAKARQAEAAERSRLRIEEEIRVVRLQLEATERQRGGAEGELQALRARAEEAEAQKRQAQEEAERLRRQVQDESQRKRQAEVELASRVKAEAEAAREKQRALQALEELRLQAEEAERRLRQAEVERARQVQVALETAQRSAEAELQSKRASFAEKTAQLERSLQEEHVAVAQLREEAERRAQQQAEAERAREEAEREL

• Full Name: plectin 1, intermediate filament binding protein 500kDa
• Calculated Molecular Weight: 532 kDa
• Observed Molecular Weight: 510 kDa
• GenBank Accession Number: NM_201380
• Gene Symbol: Plectin
• Gene ID (NCBI): 5339
• RRID: AB_3086100
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15149
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Plectin is a large (≥500-kDa) protein that is normally expressed in various tissues including skin, muscle, and brain. It binds to a number of cytoskeletal proteins including microtubules and intermediate filaments and is involved in establishment and dynamic modulation of the cytoskeletal network. In normal cells, it plays a crucial role in cytoskeleton network organization. In keratinocytes, plectin is concentrated at the basal surface, where it links intermediate filaments to the cytoplasmic domain of transmembrane glycoproteins such as integrin beta-4. (PMID: 24218614, PMID: 28281696, PMID: 23750011)