PLOD3 Monoclonal antibody, PBS Only

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  WB analysis using 60058-1-Ig (same clone as 60058-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60058-1-Ig (PLOD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 60058-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 60058-1-Ig (same clone as 60058-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 60058-1-Ig (PLOD3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60058-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 60058-1-Ig (same clone as 60058-1-PBS)

  WB result of PLOD3 antibody (60058-1-Ig, 1:2000) with si-Control and si-PLOD3 transfected HepG2 cells. This data was developed using the same antibody clone with 60058-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60058-1-Ig (same clone as 60058-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60058-1-Ig (PLOD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60058-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 60058-1-Ig (same clone as 60058-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 60058-1-Ig (PLOD3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60058-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 60058-1-Ig (same clone as 60058-1-PBS)

  IP result of anti-PLOD3 (IP:60058-1-Ig, 5ug; Detection:60058-1-Ig 1:500) with HeLa cells lysate 1400ug. This data was developed using the same antibody clone with 60058-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 60058-1-Ig (same clone as 60058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 60058-1-Ig(PLOD3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 60058-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 60058-1-Ig (same clone as 60058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 60058-1-Ig(PLOD3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 60058-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60058-1-Ig (same clone as 60058-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PLOD3 antibody (60058-1-Ig, Clone: 2G12B8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60058-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60058-1-Ig (same clone as 60058-1-PBS)

  Immunofluorescent analysis of HeLa cells using 60058-1-Ig (PLOD3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 60058-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 60058-1-Ig

  Biolayer interferometry (BLl) kinetic assays of 60058-1-Ig against Human PLOD3 were performed. The Affinity Constant is below 1 pM.

• Featured Product
• KD/KO Validated

PLOD3 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 60058-1-PBS

Clone No.2G12B8

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

2G12B8, Galactosylhydroxylysine-glucosyltransferase, LH3, Lysyl hydroxylase 3, Multifunctional procollagen lysine hydroxylase and glycosyltransferase LH3

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Product Information

60058-1-PBS targets PLOD3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1480

  Product name: Recombinant human PLOD3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 339-738 aa of BC011674

  Sequence: HEPHIADSWPQLQDHFSAVKLVGPEEALSPGEARDMAMDLCRQDPECEFYFSLDADAVLTNLQTLRILIEENRKVIAPMLSRHGKLWSNFWGALSPDEYYARSEDYVELVQRKRVGVWNVPYISQAYVIRGDTLRMELPQRDVFSGSDTDPDMAFCKSFRDKGIFLHLSNQHEFGRLLATSRYDTEHLHPDLWQIFDNPVDWKEQYIHENYSRALEGEGIVEQPCPDVYWFPLLSEQMCDELVAEMEHYGQWSGGRHEDSRLAGGYENVPTVDIHMKQVGYEDQWLQLLRTYVGPMTESLFPGYHTKARAVMNFVVRYRPDEQPSLRPHHDSSTFTLNVALNHKGLDYEGGGCRFLRYDCVISSPRKGWALLHPGRLTHYHEGLPTTWGTRYIMVSFVDP

• Full Name: procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3
• Calculated Molecular Weight: 738 aa, 85 kDa
• Observed Molecular Weight: 80-85 kDa
• GenBank Accession Number: BC011674
• Gene Symbol: PLOD3
• Gene ID (NCBI): 8985
• RRID: AB_2165779
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O60568
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PLOD3, also named as LH3, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. The major function of PLOD3 in osteoblasts is to glucosylate galactosylhydroxylysine residues in type I collagen.