PLP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

PLP2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 21482-1-AP

Synonyms

A4, A4 LSB, Intestinal membrane A4 protein, PLP2, Proteolipid protein 2

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Validation Data Gallery

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  WB analysis using 21482-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21482-1-AP (PLP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 21482-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 21482-1-AP (PLP2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 21482-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 21482-1-AP (PLP2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 21482-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 21482-1-AP (PLP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: SMMC-7721 cells, HEK-293 cells, COLO 320 cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 21482-1-AP is PLP2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PLP2 fusion protein Ag15740
• Full Name: proteolipid protein 2 (colonic epithelium-enriched)
• Calculated Molecular Weight: 152 aa, 17 kDa
• Observed Molecular Weight: 15-17 kDa
• GenBank Accession Number: BC109066
• Gene Symbol: PLP2
• Gene ID (NCBI): 5355
• RRID: AB_10858923
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Cell Physiol; The long noncoding RNA ZFAS1 promotes the progression of glioma by regulating the miR-150-5p/PLP2 axis.; Authors - Xiaojian Li ; KD Validated
human	IHC	Biomed Res Int; PLP2 Expression as a Prognostic and Therapeutic Indicator in High-Risk Multiple Myeloma.; Authors - Hua Bai