PLS3 Polyclonal antibody

• Featured Product
• KD/KO Validated

PLS3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 55216-1-AP

Synonyms

T-plastin, T-fimbrin, T plastin, Plastin-3, Plastin 3

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Validation Data Gallery

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  WB analysis of HepG2 using 55216-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55216-1-AP (PLS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 55216-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 55216-1-AP (PLS3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55216-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55216-1-AP (PLS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55216-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55216-1-AP (PLS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 55216-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55216-1-AP (PLS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 55216-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 55216-1-AP (PLS3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human oesophagus using 55216-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus using 55216-1-AP (PLS3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human oesophagus using 55216-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus using 55216-1-AP (PLS3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 55216-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55216-1-AP (PLS3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 55216-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55216-1-AP (PLS3 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HepG2 cells, COLO 320 cells, HeLa cells
• Positive IHC detected in: human oesophagus tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 2 publications below

Product Information

55216-1-AP targets PLS3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: plastin 3 (T isoform)
• Calculated Molecular Weight: 71 kDa
• Observed Molecular Weight: 63 kDa, 38 kDa
• GenBank Accession Number: NM_005032
• Gene Symbol: PLS3
• Gene ID (NCBI): 5358
• RRID: AB_10858397
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLS3, also named as T-plastin and Plastin-3, is an actin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia. It plays an important role in leukocyte function. Both T- and L-plastin are implicated in several diseases, L-plastin is considered to be a valuable marker for cancer. (PMID: 15960882) This antibody is specific to PLS3

Publications

Species	Application	Title
human	WB,IHC	J Cell Physiol; PLS3 predicts poor prognosis in pancreatic cancer and promotes cancer cell proliferation via PI3K/AKT signaling.; Authors - Zechang Xin ; KD Validated
human	WB,IHC	Environ Toxicol; PLS3 promotes papillary thyroid carcinoma progression by activating the Notch signaling pathway; Authors - Dongtao Wang ; KD Validated
human	WB	J Cell Biochem; Phosphorylation of mitochondrial phospholipid scramblase 3 by protein kinase C-delta induces its activation and facilitates mitochondrial targeting of tBid.; Authors - Yongwen He ; KD Validated
mouse	WB	Biochim Biophys Acta; Comparative proteomic analysis of mouse models of pathological and physiological cardiac hypertrophy, with selection of biomarkers of pathological hypertrophy by integrative Proteogenomics.; Authors - Hye Kyeong Kwon