Tested Applications
| Positive WB detected in | HEK-293 cells, L02 cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | mouse colon tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:9000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14452-1-AP targets PLVAP in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5963 Product name: Recombinant human PLVAP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 63-442 aa of BC056414 Sequence: TERRAEGLYSQLLGLTASQSNLTKELNFTTRAKDAIMQMWLNARRDLDRINASFRQCQGDRVIYTNNQRYMAAIILSEKQCRDQFKDMNKSCDALLFMLNQKVKTLEVEIAKEKTICTKDKESVLLNKRVAEEQLVECVKTRELQHQERQLAKEQLQKVQALCLPLDKDKFEMDLRNLWRDSIIPRSLDNLGYNLYHPLGSELASIRRACDHMPSLMSSKVEELARSLRADIERVARENSDLQRQKLEAQQGLRASQEAKQKVEKEAQAREAKLQAECSRQTQLALEEKAVLRKERDNLAKELEEKKREAEQLRMELAIRNSALDTCIKTKSQPMMPVSRPMGPVPNPQPIDPASLEEFKRKILESQRPPAGIPVAPSSG Predict reactive species |
| Full Name | plasmalemma vesicle associated protein |
| Calculated Molecular Weight | 51 kDa |
| Observed Molecular Weight | 50-60 kDa |
| GenBank Accession Number | BC056414 |
| Gene Symbol | PLVAP |
| Gene ID (NCBI) | 83483 |
| RRID | AB_2166100 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BX97 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Plasmalemma vesicle-associated protein (PLVAP) is an endothelial-associated protein that is involved in endothelial diaphragm formation and maintenance of basal vascular permeability. At the same time, PLVAP has roles in immunity by facilitating leukocyte diapedesis at inflammatory sites and controlling peripheral lymph node morphogenesis and the entry into soluble ags into lymph node conduits .(PMID: 36884119, PMID: 37810232)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PLVAP antibody 14452-1-AP | Download protocol |
| WB protocol for PLVAP antibody 14452-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PLVAP Polyclonal antibody (Cat# 14452-1-AP) has 15 citations spanning journals including Cancer Discovery, Cell Stem Cell, Neuron, Phytomedicine, Molecular Neurobiology, Frontiers in Pharmacology, Scientific Reports, iScience, Journal of Virology, Microvascular Research, Biochemical and Biophysical Research Communications, bioRxiv, and Cell Signaling. Research fields cover tumor microenvironment, blood-brain/blood-retinal barrier biology, Wnt signaling, intestinal barrier dysfunction, endothelial permeability, ischemic stroke, diabetic retinopathy, viral infection, hearing loss, and pulmonary edema.
| Species | Application | Title |
|---|---|---|
Cancer Discov Single-Cell View of Tumor Microenvironment Gradients in Pleural Mesothelioma. | ||
Cell Stem Cell Modeling blood-brain barrier formation and cerebral cavernous malformations in human PSC-derived organoids | ||
Neuron Glutamatergic neuronal activity regulates angiogenesis and blood-retinal barrier maturation via Norrin/β-catenin signaling | ||
Neuron The Wnt Inhibitor Apcdd1 Coordinates Vascular Remodeling and Barrier Maturation of Retinal Blood Vessels. | ||
Phytomedicine Formononetin and daidzein from Huoxiangzhengqi formula ameliorate Salmonella Typhimurium-induced intestinal flora dysbiosis and barrier dysfunction. | ||
Mol Neurobiol Canonical Wnt Pathway Maintains Blood-Brain Barrier Integrity upon Ischemic Stroke and Its Activation Ameliorates Tissue Plasminogen Activator Therapy. |
Reviews
What customers say
The single review rates the antibody 5 stars for Western Blot. The user reports excellent performance for PLVAP detection in cultured bEnd5 cells, noting strong and specific bands even at a relatively high dilution of 1:4500. Overall, the feedback highlights reliable specificity and sensitivity suitable for high-dilution WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Michelle (Verified Customer) (12-01-2023) | This antibody works very well for PLVAP westernblot analysis. We detected strong und specific PLVAP bands, even with this relatively high dilution of 1:4500.
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