PLVAP Monoclonal antibody

PLVAP Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D4A6

Cat no : 67158-1-Ig

Synonyms

FELS, gp68, Plasmalemma vesicle protein 1, PLVAP, PV 1, PV1

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Validation Data Gallery

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  WB analysis of human placenta using 67158-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67158-1-Ig (PLVAP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67158-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67158-1-Ig (PLVAP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 67158-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67158-1-Ig (PLVAP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 67158-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67158-1-Ig (PLVAP antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human placenta tissue, pig spleen tissue, pig kidney tissue
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67158-1-Ig targets PLVAP in WB, IHC, ELISA applications and shows reactivity with Human, Pig samples.

• Tested Reactivity: Human, Pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: PLVAP fusion protein Ag6359
• Full Name: plasmalemma vesicle associated protein
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC056414
• Gene Symbol: PLVAP
• Gene ID (NCBI): 83483
• RRID: AB_2882455
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.