PLVAP Monoclonal antibody

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  WB analysis of human placenta using 67158-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67158-1-Ig (PLVAP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67158-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67158-1-Ig (PLVAP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 67158-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67158-1-Ig (PLVAP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 67158-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67158-1-Ig (PLVAP antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse small intestine using 67158-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse small intestine tissue using PLVAP antibody (67158-1-Ig, Clone: 1D4A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

PLVAP Monoclonal Antibody for WB, IHC, IF-Fro, ELISA

Cat No. 67158-1-Ig

Clone No.1D4A6

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, IF-Fro, ELISA

Synonyms

1D4A6, FELS, Fenestrated endothelial-linked structure protein, gp68, Plasmalemma vesicle-associated protein

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Tested Applications

• Positive WB detected in: human placenta tissue, pig spleen tissue, pig kidney tissue
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-Fro detected in: mouse small intestine tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67158-1-Ig targets PLVAP in WB, IHC, IF-Fro, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: PLVAP fusion protein Ag6359
• Full Name: plasmalemma vesicle associated protein
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC056414
• Gene Symbol: PLVAP
• Gene ID (NCBI): 83483
• RRID: AB_2882455
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BX97
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Plasmalemma vesicle-associated protein (PLVAP) is an endothelial-associated protein that is involved in endothelial diaphragm formation and maintenance of basal vascular permeability. At the same time, PLVAP has roles in immunity by facilitating leukocyte diapedesis at inflammatory sites and controlling peripheral lymph node morphogenesis and the entry into soluble ags into lymph node conduits .(PMID: 36884119, PMID: 37810232)