PLVAP Monoclonal antibody, PBS Only

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  WB analysis of human placenta using 67158-1-Ig (same clone as 67158-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67158-1-Ig (PLVAP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67158-1-PBS in a different storage buffer formulation.

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  WB analysis using 67158-1-Ig (same clone as 67158-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67158-1-Ig (PLVAP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67158-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67158-1-Ig (same clone as 67158-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67158-1-Ig (PLVAP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67158-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67158-1-Ig (same clone as 67158-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67158-1-Ig (PLVAP antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67158-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse small intestine using 67158-1-Ig (same clone as 67158-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse small intestine tissue using PLVAP antibody (67158-1-Ig, Clone: 1D4A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67158-1-PBS in a different storage buffer formulation.

PLVAP Monoclonal Antibody for WB, IHC, IF-Fro, Indirect ELISA

Cat No. 67158-1-PBS

Clone No.1D4A6

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, IF-Fro, Indirect ELISA

Synonyms

1D4A6, FELS, Fenestrated endothelial-linked structure protein, gp68, Plasmalemma vesicle-associated protein

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Product Information

67158-1-PBS targets PLVAP in WB, IHC, IF-Fro, Indirect ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: PLVAP fusion protein Ag6359
• Full Name: plasmalemma vesicle associated protein
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC056414
• Gene Symbol: PLVAP
• Gene ID (NCBI): 83483
• RRID: AB_2882455
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BX97
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Plasmalemma vesicle-associated protein (PLVAP) is an endothelial-associated protein that is involved in endothelial diaphragm formation and maintenance of basal vascular permeability. At the same time, PLVAP has roles in immunity by facilitating leukocyte diapedesis at inflammatory sites and controlling peripheral lymph node morphogenesis and the entry into soluble ags into lymph node conduits .(PMID: 36884119, PMID: 37810232)