CoraLite® Plus 647 Anti-Mouse PLVAP (MECA-32) Rat IgG2a Recombinant Antibody

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  FC experiment of bEnd.3 using CL647-65664

  1x10^6 bEnd.3 cells were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse PLVAP (MECA-32) Rat IgG2a RecAb (CL647-65664, Clone: MECA-32) (red) or CoraLite® Plus 647 Rat IgG2a Isotype Control (2A3) (CL647-65209, Clone: 2A3) (blue). Cells were not fixed.

PLVAP Uni-rAb^TM Recombinant Antibody for FC

Cat No. CL647-65664

Clone No.MECA-32

Host / Isotype

Rat / IgG2a

Reactivity

mouse

Applications

FC

Synonyms

MECA32, MECA-32, Panendothelial cell antigen, Plasmalemma vesicle protein 1, PV 1

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Tested Applications

• Positive FC detected in: bEnd.3 cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65664 targets PLVAP in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: N/A
• Full Name: plasmalemma vesicle associated protein
• GenBank Accession Number: BC013517
• Gene Symbol: Plvap
• Gene ID (NCBI): 84094
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Plasmalemma vesicle-associated protein (PLVAP), also known as PV-1, PAL-E and MECA-32 antigen, is an endothelial cell-specific type II membrane N-glycosylated glycoprotein with a molecular weight of approximately 50-65 kDa, that forms homodimers in situ (PMID: 7626790; 30231925). The MECA-32 antibody, derived from rats immunized with murine lymph node stroma, shows high specificity for mouse endothelium in both embryonic and mature tissues (PMID: 2683264; 7626790). It recognizes most endothelial cells in the embryonic and in the adult mouse, with the exception of the brain, skeletal, and cardiac muscle, where the MECA-32 antigen has a more restricted distribution. In skeletal and cardiac muscle only small arterioles and venules express the MECA-32 antigen, while in the brain its expression is negatively correlated with the differentiation of the vasculature to form the blood brain barrier (PMID: 7626790).