FITC Plus Anti-Mouse PLVAP (MECA-32)

PLVAP Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

MECA-32

Cat no : FITC-65214

Synonyms

MECA-32, PLVAP, PV 1

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Validation Data Gallery

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  FC experiment of bEnd.3 using FITC-65214

  1X10^6 bEnd.3 cells were surface stained with 1 ug FITC Plus Anti-Mouse PLVAP (FITC-65214, Clone: MECA-32) (red) or 1 ug Control Antibody. Cells were not fixed.

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Tested Applications

• Positive FC detected in: bEnd.3 cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-65214 targets PLVAP in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mouse lymph node stromal cells
• Full Name: plasmalemma vesicle associated protein
• GenBank Accession Number: BC013517
• Gene Symbol: Plvap
• Gene ID (NCBI): 84094
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Plasmalemma vesicle-associated protein (PLVAP), also known as PV-1, PAL-E and MECA-32 antigen, is an endothelial cell-specific type II membrane N-glycosylated glycoprotein with a molecular weight of approximately 50-65 kDa, that forms homodimers in situ (PMID: 7626790; 30231925). The MECA-32 antibody, derived from rats immunized with murine lymph node stroma, shows high specificity for mouse endothelium in both embryonic and mature tissues (PMID: 2683264; 7626790). It recognizes most endothelial cells in the embryonic and in the adult mouse, with the exception of the brain, skeletal, and cardiac muscle, where the MECA-32 antigen has a more restricted distribution. In skeletal and cardiac muscle only small arterioles and venules express the MECA-32 antigen, while in the brain its expression is negatively correlated with the differentiation of the vasculature to form the blood brain barrier (PMID: 7626790).