Tested Applications
| Positive FC detected in | bEnd.3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
FITC-65214 targets PLVAP in FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Mouse lymph node stromal cells Predict reactive species |
| Full Name | plasmalemma vesicle associated protein |
| GenBank Accession Number | BC013517 |
| Gene Symbol | Plvap |
| Gene ID (NCBI) | 84094 |
| RRID | AB_2924137 |
| Conjugate | FITC Plus Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 495 nm / 524 nm |
| Excitation Laser | Blue laser (488 nm) |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q91VC4 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Plasmalemma vesicle-associated protein (PLVAP), also known as PV-1, PAL-E and MECA-32 antigen, is an endothelial cell-specific type II membrane N-glycosylated glycoprotein with a molecular weight of approximately 50-65 kDa, that forms homodimers in situ (PMID: 7626790; 30231925). The MECA-32 antibody, derived from rats immunized with murine lymph node stroma, shows high specificity for mouse endothelium in both embryonic and mature tissues (PMID: 2683264; 7626790). It recognizes most endothelial cells in the embryonic and in the adult mouse, with the exception of the brain, skeletal, and cardiac muscle, where the MECA-32 antigen has a more restricted distribution. In skeletal and cardiac muscle only small arterioles and venules express the MECA-32 antigen, while in the brain its expression is negatively correlated with the differentiation of the vasculature to form the blood brain barrier (PMID: 7626790).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for FITC Plus PLVAP antibody FITC-65214 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The antibody is Anti-Mouse PLVAP (MECA-32), catalog number FITC-65214. It has a total of 1 citation. The citation appears in Cell Reports (2024), a study investigating how macrophages preserve endothelial cell specialization in the adrenal gland to modulate aldosterone secretion and blood pressure. The associated research fields include immunology, cardiovascular biology, and endocrinology. The application used was flow cytometry (FC) in mouse samples.
| Species | Application | Title |
|---|---|---|
Cell Rep Macrophages preserve endothelial cell specialization in the adrenal gland to modulate aldosterone secretion and blood pressure |
