PLXDC2 Recombinant monoclonal antibody, PBS Only

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  WB analysis of MKN-45 using 83449-4-RR (same clone as 83449-4-PBS)

  MKN-45 cells were subjected to SDS PAGE followed by western blot with 83449-4-RR (PLXDC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83449-4-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 83449-4-RR (same clone as 83449-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83449-4-RR (PLXDC2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83449-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 83449-4-RR (same clone as 83449-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83449-4-RR (PLXDC2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83449-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83449-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83449-4-RR against Human PLXDC2 were performed. The affinity constant is 42.5 pM.

PLXDC2 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 83449-4-PBS

Clone No.240174A11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

UNQ2514/PRO6003, Tumor endothelial marker 7-related protein, TEM7R, Plexin domain-containing protein 2, plexin domain containing 2

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Product Information

83449-4-PBS targets PLXDC2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2940

  Product name: Recombinant human PLXDC2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 28-407 aa of BC012885

  Sequence: ADGKPGDQILDWQYGVTQAFPHTEEEVEVDSHAYSHRWKRNLDFLKAVDTNRASVGQDSPEPRSFTDLLLDDGQDNNTQIERVNLSFDFPFYGHFLREITVATGGFIYTGEVVHRMLTATQYIAPLMANFDPSVSRNSTVRYFDNGTALVVQWDHVHLQDNYNLGSFTFQATLLMDGRIIFGYKEIPVLVTQISSTNHPVKVGLSDAFVVVHRIQQIPNVRRRTIYEYHRVELQMSKITNISAVEMTPLPTCLQFNRCGPCVSSQIGFNCSWCSKLQRCSSGFDRHRQDWVDSGCPEESKEKMCENTEPVETSSRTTTTVGATTTQFRVLTTTRRAVTSQFPTSLPTEDDTKIALHLKDNGASTDDSAAEKKGGTLHAGL

• Full Name: plexin domain containing 2
• Calculated Molecular Weight: 480 aa, 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC012885
• Gene Symbol: PLXDC2
• Gene ID (NCBI): 84898
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q6UX71
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PLXDC2 (Plexin-domain containing 2), also named tumor endothelial marker 7-related protein (TEM7R), is a single-pass type I membrane protein with some homology to nidogen and to plexins. PLXDC2 is located in tumor endothelium and plays a role in tumor angiogenesis. Three isoforms of PLXDC2 produced by alternative splicing have been described. Recent finding revealed that PLXDC2 is a mitogen for neural progenitors and functions as a novel regulator of the development of different brain regions, suggesting its role in coordinating proliferation and differentiation in the developing nervous system.