PLZF Monoclonal antibody

PLZF Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat and More (1)

Applications

WB, ELISA, IF

Conjugate

Unconjugated

Publications

4

CloneNo.

2E6F12

Cat no : 66672-1-Ig

Synonyms

PLZF, ZBTB16, Zinc finger protein 145, Zinc finger protein PLZF, ZNF145

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Validation Data Gallery

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  WB analysis of Ramos using 66672-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 66672-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Ramos using 66672-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 66672-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Daudi using 66672-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66672-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 66672-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Ramos cells, Raji cells, Daudi cells, HeLa cells, THP-1 cells, human testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below

Product Information

66672-1-Ig targets PLZF in WB, ELISA, IF applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PLZF fusion protein Ag25180
• Full Name: zinc finger and BTB domain containing 16
• Calculated Molecular Weight: 673 aa, 74 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC026902
• Gene Symbol: PLZF
• Gene ID (NCBI): 7704
• RRID: AB_2882026
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLZF, also named as Zinc finger protein 145, is a 673 amino acid protein, which belongs to the krueppel C2H2-type zinc-finger protein family. PLZF is expressed in bone marrow, early myeloid cell lines and peripheral blood mononuclear cells. PLZF may play a role in myeloid maturation and in the development and/or maintenance of other differentiated tissues.

Publications

Species	Application	Title
mouse	WB	Front Physiol; Abscopal effects of thoracic X-ray radiation on spermatogenesis in mice; Authors - Pan-Pan Lai
mouse	WB	iScience; Chromodomain helicase DNA-binding domain 2 maintains spermatogonial self-renewal by promoting chromatin accessibility and mRNA stability; Authors - Ziqian Min
mouse	WB	J Hazard Mater; Prenatal and postnatal exposure to polystyrene microplastics induces testis developmental disorder and affects male fertility in mice; Authors - Tianxin Zhao
mouse	IF	Med Microbiol Immunol; Effects of acetate-producing Blautia wexlerae on oxidative stress and NLRP3 inflammasome in obesity-associated male infertility; Authors - Yucheng Zhong