Tested Applications
| Positive WB detected in | Ramos cells, Raji cells, Daudi cells, HeLa cells, THP-1 cells, human testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66672-1-Ig targets PLZF in WB, IF, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25180 Product name: Recombinant human PLZF protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 101-400 aa of BC026902 Sequence: DLLYAAEILEIEYLEEQCLKMLETIQASDDNDTEATMADGGAEEEEDRKARYLKNIFISKHSSEESGYASVAGQSLPGPMVDQSPSVSTSFGLSAMSPTKAAVDSLMTIGQSLLQGTLQPPAGPEEPTLAGGGRHPGVAEVKTEMMQVDEVPSQDSPGAAESSISGGMGDKVEERGKEGPGTPTRSSVITSARELHYGREESAEQVPPPAEAGQAPTGRPEHPAPPPEKHLGIYSVLPNHKADAVLSMPSSVTSGLHVQPALAVSMDFSTYGGLLPQGFIQRELFSKLGELAVGMKSESR Predict reactive species |
| Full Name | zinc finger and BTB domain containing 16 |
| Calculated Molecular Weight | 673 aa, 74 kDa |
| Observed Molecular Weight | 75 kDa |
| GenBank Accession Number | BC026902 |
| Gene Symbol | PLZF |
| Gene ID (NCBI) | 7704 |
| RRID | AB_2882026 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q05516 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PLZF, also named as Zinc finger protein 145, is a 673 amino acid protein, which belongs to the krueppel C2H2-type zinc-finger protein family. PLZF is expressed in bone marrow, early myeloid cell lines and peripheral blood mononuclear cells. PLZF may play a role in myeloid maturation and in the development and/or maintenance of other differentiated tissues.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PLZF antibody 66672-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PLZF Monoclonal antibody (Cat# 66672-1-Ig) has 10 total citations. Citations appear in journals including Phytomedicine, Journal of Hazardous Materials, Journal of Medicinal Chemistry, Ecotoxicology and Environmental Safety, Biomedical Journal, iScience, Frontiers in Physiology, Andrology, Medical Microbiology and Immunology, and Journal of Genetics and Genomics. Research fields span reproductive biology and spermatogenesis, male infertility, ferroptosis, osteoporosis, hematological cancers, radiation effects, and microbiota-metabolism studies, with applications mainly in Western blot and immunofluorescence.
| Species | Application | Title |
|---|---|---|
Phytomedicine Guilu Erxian glue mitigates spermatogenesis dysfunction through HIF-1α/SLC7A11-mediated ferroptosis inhibition: An integrated metabolomics and network pharmacology study. | ||
J Hazard Mater Prenatal and postnatal exposure to polystyrene microplastics induces testis developmental disorder and affects male fertility in mice | ||
J Med Chem Discovery of Novel Potent Triple IKZF1/2/3 Degraders for the Treatment of Hematological Cancers | ||
Ecotoxicol Environ Saf Ameliorating effects of rTMS on spermatogenesis disorder caused by cranial radiation-induced abscopal effects. | ||
Biomed J Autophagy-induced osteoblast-derived exosomes maintain bone formation and prevent osteoporosis by remodeling gut microbiota-metabolism | ||
iScience Chromodomain helicase DNA-binding domain 2 maintains spermatogonial self-renewal by promoting chromatin accessibility and mRNA stability |
