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- KD/KO Validated
PMEPA1 Polyclonal antibody
PMEPA1 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 16521-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | L02 cells, U2OS cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 5 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
16521-1-AP targets PMEPA1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PMEPA1 fusion protein Ag9744 |
| Full Name | prostate transmembrane protein, androgen induced 1 |
| Calculated Molecular Weight | 287 aa, 32 kDa |
| Observed Molecular Weight | 32-36 kDa |
| GenBank Accession Number | BC015918 |
| Gene Symbol | PMEPA1 |
| Gene ID (NCBI) | 56937 |
| RRID | AB_2284052 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PMEPA1, also known as TMEPAI, was first discovered as androgen inducible gene in hormone responsive LNCaP cells. PMEPA1 is highly expressed in various solid tumors and is known to play important roles in the transforming growth factor-β(TGF-β) signaling pathway (PMID: 27697531).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PMEPA1 antibody 16521-1-AP | Download protocol |
| IHC protocol for PMEPA1 antibody 16521-1-AP | Download protocol |
| IF protocol for PMEPA1 antibody 16521-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO Mol Med An autocrine ActivinB mechanism drives TGFβ/Activin signaling in Group 3 medulloblastoma. | ||
Front Immunol PMEPA1 Is a Prognostic Biomarker That Correlates With Cell Malignancy and the Tumor Microenvironment in Bladder Cancer
| ||
FASEB J PMEPA1 and NEDD4 control the proton production of osteoclasts by regulating vesicular trafficking.
| ||
J Virol NF-κB-Interacting Long Noncoding RNA Regulates HIV-1 Replication and Latency by Repressing NF-κB Signaling. | ||
Biochem Biophys Res Commun PMEPA1, a TGF-β- and hypoxia-inducible gene that participates in hypoxic gene expression networks in solid tumors.
| ||
Genes Cancer Increased Smad3 and reduced Smad2 levels mediate the functional switch of TGF-β from growth suppressor to growth and metastasis promoter through TMEPAI/PMEPA1 in triple negative breast cancer. |
