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  • KD/KO Validated

PMEPA1 Polyclonal antibody

PMEPA1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 16521-1-AP

Synonyms

PMEPA1, STAG1, TMEPAI



Tested Applications

Positive WB detected inL02 cells, DU 145 cells
Positive IHC detected inhuman prostate cancer tissue, mouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

16521-1-AP targets PMEPA1 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PMEPA1 fusion protein Ag9744
Full Name prostate transmembrane protein, androgen induced 1
Calculated molecular weight 287 aa, 32 kDa
Observed molecular weight 32-36 kDa
GenBank accession numberBC015918
Gene symbol PMEPA1
Gene ID (NCBI) 56937
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PMEPA1, also known as TMEPAI, was first discovered as androgen inducible gene in hormone responsive LNCaP cells. PMEPA1 is highly expressed in various solid tumors and is known to play important roles in the transforming growth factor-β(TGF-β) signaling pathway (PMID: 27697531).

Protocols

Product Specific Protocols
WB protocol for PMEPA1 antibody 16521-1-APDownload protocol
IHC protocol for PMEPA1 antibody 16521-1-APDownload protocol
IF protocol for PMEPA1 antibody 16521-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Genes Cancer

Increased Smad3 and reduced Smad2 levels mediate the functional switch of TGF-β from growth suppressor to growth and metastasis promoter through TMEPAI/PMEPA1 in triple negative breast cancer.

Authors - Prajjal K Singha
humanWB

Biochem Biophys Res Commun

PMEPA1, a TGF-β- and hypoxia-inducible gene that participates in hypoxic gene expression networks in solid tumors.

Authors - Masaru Koido
  • KD Validated
humanWB

J Virol

NF-κB-Interacting Long Noncoding RNA Regulates HIV-1 Replication and Latency by Repressing NF-κB Signaling.

Authors - Hong Wang
mouseIF

FASEB J

PMEPA1 and NEDD4 control the proton production of osteoclasts by regulating vesicular trafficking.

Authors - Hirohito Hirata
  • KO Validated
humanWB

EMBO Mol Med

An autocrine ActivinB mechanism drives TGFβ/Activin signaling in Group 3 medulloblastoma.

Authors - Morgane Morabito