PMM2 Polyclonal antibody

• Featured Product
• KD/KO Validated

PMM2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 10666-1-AP

Synonyms

phosphomannomutase 2, EC:5.4.2.8

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Validation Data Gallery

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  WB analysis of multi-tissue using 10666-1-AP

  WB results of 10666-1-AP (PMM2 antibody) on several lysates.

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  WB analysis of HepG2 using 10666-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10666-1-AP (PMM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10666-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10666-1-AP (PMM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 10666-1-AP

  IP result of anti-PMM2 (IP:10666-1-AP, 4ug; Detection:10666-1-AP 1:1000) with mouse lung tissue lysate 3600ug.

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  IHC staining of human breast cancer using 10666-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10666-1-AP (PMM2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10666-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10666-1-AP (PMM2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10666-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10666-1-AP (PMM2 antibody) at dilution of 1:100 (under 25x lens).

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  IF Staining of HepG2 using 10666-1-AP

  Immunofluorescent analysis of HepG2 cells using 10666-1-AP (PMM2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, multi-tissue
• Positive IP detected in: mouse lung tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

10666-1-AP targets PMM2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PMM2 fusion protein Ag1031
• Full Name: phosphomannomutase 2
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 28-30 kDa
• GenBank Accession Number: BC008310
• Gene Symbol: PMM2
• Gene ID (NCBI): 5373
• RRID: AB_2166973
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PMM2(Phosphomannomutase 2) belongs to the eukaryotic PMM family. It is involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. PMM2 catalyzes the second step in the conversion of fructose-6P to GDP-mannose.

Publications

Species	Application	Title
human	WB	Thromb Haemost; N-Glycosylation Deficiency Reduces the Activation of Protein C and Disrupts Endothelial Barrier Integrity; Authors - Tiffany Pascreau ; KD Validated
mouse	WB	J Proteome Res; Changes in the striatal proteome of YAC128Q mice exhibit gene-environment interactions between mutant huntingtin and manganese.; Authors - Wegrzynowicz Michal M
human	WB	Mol Genet Metab; Complex metabolic disharmony in PMM2-CDG paves the way to new therapeutic approaches; Authors - Nastassja Himmelreich
human	WB	J Inherit Metab Dis; Lack of phosphomannomutase 2 affects Xenopus laevis morphogenesis and the non-canonical Wnt5a/Ror2 signalling.; Authors - Nastassja Himmelreich
human	WB	Dis Model Mech; Repurposing the aldose reductase inhibitor and diabetic neuropathy drug epalrestat for the congenital disorder of glycosylation PMM2-CDG.; Authors - Sangeetha Iyer
	WB	PLoS One; Heterodimerization of Two Pathological Mutants Enhances the Activity of Human Phosphomannomutase2.; Authors - Giuseppina Andreotti