PMPCA Polyclonal antibody
PMPCA Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 26536-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | MDA-MB-453s cells, T-47D cells |
Positive IP detected in | MDA-MB-453s cells |
Positive IF/ICC detected in | T-47D cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 2 publications below |
Product Information
The immunogen of 26536-1-AP is PMPCA Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | PMPCA fusion protein Ag19206 |
Full Name | peptidase (mitochondrial processing) alpha |
Calculated Molecular Weight | 525 aa, 58 kDa |
Observed Molecular Weight | 50-58 kDa |
GenBank Accession Number | BC132724 |
Gene Symbol | PMPCA |
Gene ID (NCBI) | 23203 |
RRID | AB_2880545 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for PMPCA antibody 26536-1-AP | Download protocol |
IF protocol for PMPCA antibody 26536-1-AP | Download protocol |
IP protocol for PMPCA antibody 26536-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Hum Mol Genet GRP75 overexpression rescues frataxin deficiency and mitochondrial phenotypes in Friedreich Ataxia cellular models. |