PMPCA Polyclonal antibody

PMPCA Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 26536-1-AP

Synonyms

Alpha MPP, INPP5E, KIAA0123, MPPA, P 55, PMPCA



Tested Applications

Positive WB detected inMDA-MB-453s cells, T-47D cells
Positive IP detected inMDA-MB-453s cells
Positive IF/ICC detected inT-47D cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 26536-1-AP is PMPCA Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PMPCA fusion protein Ag19206
Full Name peptidase (mitochondrial processing) alpha
Calculated Molecular Weight 525 aa, 58 kDa
Observed Molecular Weight 50-58 kDa
GenBank Accession NumberBC132724
Gene Symbol PMPCA
Gene ID (NCBI) 23203
RRIDAB_2880545
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PMPCA antibody 26536-1-APDownload protocol
IF protocol for PMPCA antibody 26536-1-APDownload protocol
IP protocol for PMPCA antibody 26536-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Chem Biol

Enhanced mapping of small-molecule binding sites in cells

Authors - Jacob M Wozniak
humanWB

Hum Mol Genet

GRP75 overexpression rescues frataxin deficiency and mitochondrial phenotypes in Friedreich Ataxia cellular models.

Authors - Yi Na Dong