PMPCA Polyclonal antibody

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  WB analysis using 26536-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26536-1-AP (PMPCA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of T-47D using 26536-1-AP

  T-47D cells were subjected to SDS PAGE followed by western blot with 26536-1-AP (PMPCA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of MDA-MB-453s using 26536-1-AP

  IP result of anti-PMPCA (IP:26536-1-AP, 4ug; Detection:26536-1-AP 1:500) with MDA-MB-453s cells lysate 2800ug.

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  IF Staining of T-47D using 26536-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed T-47D cells using 26536-1-AP (PMPCA antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

PMPCA Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 26536-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Synonyms

Alpha MPP, INPP5E, KIAA0123, MPPA, P 55, PMPCA

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Tested Applications

• Positive WB detected in: MDA-MB-453s cells, T-47D cells
• Positive IP detected in: MDA-MB-453s cells
• Positive IF/ICC detected in: T-47D cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

26536-1-AP targets PMPCA in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19206

  Product name: Recombinant human PMPCA protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 176-525 aa of BC132724

  Sequence: LTDEEVEMTRMAVQFELEDLNLRPDPEPLLTEMIHEAAYRENTVGLHRFCPTENVAKINREVLHSYLRNYYTPDRMVLAGVGVEHEHLVDCARKYLLGVQPAWGSAEAVDIDRSVAQYTGGIAKLERDMSNVSLGPTPIPELTHIMVGLESCSFLEEDFIPFAVLNMMMGGGGSFSAGGPGKGMFSRLYLNVLNRHHWMYNATSYHHSYEDTGLLCIHASADPRQVREMVEIITKEFILMGGTVDTVELERAKTQLTSMLMMNLESRPVIFEDVGRQVLATRSRKLPHELCTLIRNVKPEDVKRVASKMLRGKPAVAALGDLTDLPTYEHIQTALSSKDGRLPRTYRLFR

• Full Name: peptidase (mitochondrial processing) alpha
• Calculated Molecular Weight: 525 aa, 58 kDa
• Observed Molecular Weight: 50-58 kDa
• GenBank Accession Number: BC132724
• Gene Symbol: PMPCA
• Gene ID (NCBI): 23203
• RRID: AB_2880545
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q10713
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Chem Biol; Enhanced mapping of small-molecule binding sites in cells; Authors - Jacob M Wozniak
human	WB	Hum Mol Genet; GRP75 overexpression rescues frataxin deficiency and mitochondrial phenotypes in Friedreich Ataxia cellular models.; Authors - Yi Na Dong