Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18266-1-AP targets Pinin in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6643 Product name: Recombinant human PNN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 376-717 aa of BC062602 Sequence: EKQQDSQPEEVMDVLEMVENVKHVIADQEVMETNRVESVEPSENEASKELEPEMEFEIEPDKECKSLSPGKENVSALDMEKESEEKEEKESEPQPEPVAQPQPQSQPQLQLQSQSQPVLQSQPPSQPEDLSLAVLQPTPQVTQEQGHLLPERKDFPVESVKLTEVPVEPVLTVHPESKSKTKTRSRSRGRARNKTSKSRSRSSSSSSSSSSSTSSSSGSSSSSGSSSSRSSSSSSSSTSGSSSRDSSSSTSSSSESRSRSRGRGHNRDRKHRRSVDRKRRDTSGLERSHKSSKGGSSRDTKGSKDKNSRSDRKRSISESSRSGKRSSRSERDRKSDRKDKRR Predict reactive species |
| Full Name | pinin, desmosome associated protein |
| Calculated Molecular Weight | 82 kDa |
| Observed Molecular Weight | 82 kDa, 140 kDa |
| GenBank Accession Number | BC062602 |
| Gene Symbol | Pinin |
| Gene ID (NCBI) | 5411 |
| RRID | AB_10642138 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H307 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PNN, also named as DRS or 140 kDa nuclear and cell adhesion-related phosphoprotein, is a 717 amino acid protein, which belongs to the pinin family. PNN localizes in the plasma membrane and is expressed in placenta, lung, liver, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon, heart, epidermis, esophagus, brain and smooth and skeletal muscle. PNN is expressed strongly in melanoma metastasis lesions and advanced primary tumors. PNN as a transcriptional activator binds to the E-box 1 core sequence of the E-cadherin promoter gene. PNN is involved in the establishment and maintenance of epithelia cell-cell adhesion. PNN is a potential tumor suppressor for renal cell carcinoma. The calculated molecular weight of PNN is 82 kDa (native form), but the post-modified protein is 140 kDa (phospho form of isoform 1).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Pinin antibody 18266-1-AP | Download protocol |
| IP protocol for Pinin antibody 18266-1-AP | Download protocol |
| WB protocol for Pinin antibody 18266-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Pinin Polyclonal antibody (catalog no. 18266-1-AP) has 10 citations. They appear in journals including Cell Metabolism, Science Advances, Cell Reports, Oncotarget, iScience, Molecular Oncology, Experimental Physiology, and bioRxiv. Research fields span cancer biology (breast, lung, nasopharyngeal, colon, and hepatocellular carcinoma), RNA metabolism and m6A modification, neurodegenerative diseases (ALS, TDP-43, tau pathology), and RNA processing/export.
| Species | Application | Title |
|---|---|---|
Cell Metab NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis. | ||
Sci Adv M6A-dependent RNA condensation underlies FUS autoregulation and can be harnessed for ALS therapy development | ||
Cell Rep Stress-induced TDP-43 nuclear condensation causes splicing loss of function and STMN2 depletion | ||
Oncotarget Quantitative proteomics reveals molecular mechanism of gamabufotalin and its potential inhibition on Hsp90 in lung cancer. | ||
iScience C9orf72 poly-PR forms anisotropic condensates causative of nuclear TDP-43 pathology | ||
Mol Oncol LncRNA AATBC regulates Pinin to promote metastasis in nasopharyngeal carcinoma.
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Reviews
What customers say
The product received a 5-star rating from a researcher who used it for immunofluorescence on HeLa cells at a 1:1000 dilution. The antibody was incubated for 2 hours at room temperature in 5% goat serum in PBST and produced very good signal, clearly labeling nuclear speckles. Overall, the reviewer reported excellent performance and strong staining results.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Tatyana (Verified Customer) (05-14-2023) | ICC on HeLa cells; antibody diluted in 5% goat serum in PBST; 2 h at RT temperature incubation. Labels nuclear speckles. Very good signal
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